Recombinant Human Collagen IV alpha 1/Arresten Protein, CF

Recombinant Human Collagen IV alpha 1 His-tag Protein (Catalog # 11597-CL) binds Recombinant Human Integrin alpha 1 beta 1 (7064-AB) with an ED50 of 85.0‑850 ng/mL.

2 μg/lane of Recombinant Human Collagen IV alpha 1 His-tag Protein (Catalog # 11597-CL) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 22‑33 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Collagen IV alpha 1/Arresten Protein, CF Summary

• Additional Information: His-tag
• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human Collagen IV alpha 1 His-tag (Catalog # 11597-CL) binds Recombinant Human Integrin alpha 1 beta 1 (Catalog # 7064-AB) with an ED₅₀ of 85.0-850 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Collagen IV alpha 1 protein
  Ser1441-Thr1669, with a C-ter 6xHis tag
• Accession #: P02462.3
• N-terminal Sequence: Ser 1441
• Protein/Peptide Type: Recombinant Enzymes
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 22-33 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Collagen IV alpha 1/Arresten Protein, CF

• alpha-1 chain
• Arresten
• COL4A1 NC1 domain
• COL4A1
• collagen alpha-1(IV) chain
• Collagen IV alpha 1
• collagen IV, alpha-1 polypeptide
• collagen, type IV, alpha 1
• Collagen-4
• EC 3.4.23
• EC 6.3.1.2

Background

Type IV collagen is a member of the collagen superfamily.  Unlike other collagens, type IV occurs only in basement membranes. In mammals, type IV collagen has up to 6 genetically distinct alpha chains, designated alpha-1(IV) to alpha-6(IV), each of which can form a triple helix structure with two other chains (1-3).  Each chain contains three domains: a cysteine- and lysine-rich N-terminal domain, a collagenous triple repeat region, and a non-collagenous (NC1) C-terminal domain (4). The cysteine and lysine residues at the amino-terminal domain are essential for interchain crosslinking (5). Arresten, which comprise the C-terminal NC1 domain, has anti-angiogenic activity and inhibits tumor formation (6-7).  Within this region, human Arresten shares 97% and 75% sequence identity with mouse and rat Arresten, respectively.

1. Myllyharju, J. and KI Kivirikko (2004) Trends Genet. 20:33.
2. Veit, G. et al. (2006) J. Biol. Chem. 281:3494.
3. Guey, S. and D. Herve (2022) Cereb. Circ. Cogn. Behav. 3:100140.
4. Hudson, B.G., et al. (2003) N. Engl. J. Med. 348:2543.
5. Than, M.E. et al. (2002) Proc. Natl. Acad. Sci. USA 99:6607.
6. Nyberg, P. et al. (2008) Exp. Cell Res. 314:3292.
7. Colorado, P.C. et al. (2000) Cancer Res. 60:2520.

Bioinformatics

• Uniprot:
  • P02462.3()