Recombinant Human CLC/CNTFR alpha Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CLC/CNTFR alpha Chimera Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 10-25 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human CLC/CNTF R alpha Chimera protein
  

  Human CNTF R alpha (Gln23 - Pro346) Accession # P26992	GSGSSRGGSGSGGSGGGGSKL	Human CLC (Leu28 - Phe225) Accession # Q9UBD9	6-His tag
  N-terminus		C-terminus

• Accession #: Q9UBD9 (CLC) & P26992 (CNTF R alpha )
• N-terminal Sequence: No results obtained: Gln23 predicted
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-93 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CLC/CNTFR alpha Chimera Protein, CF

• CLC/CNTF R alpha Chimera
• CNTFR

Background

Cardiotrophin-like cytokine (CLC), (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3) is a 22 - 25 kDa member of the IL-6 family of cytokines (1 - 3). As such, it is expressed as a long, type I cytokine with four alpha -helices in its structure (2). Human CLC is synthesized as a 225 amino acid (aa) precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for Ciliary Neurotrophic Factor Receptor alpha (CNTF R alpha ) and Cytokine Receptor-like Factor (CLF) (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently complexed to either CLF or soluble CNTF R alpha (5, 6). Once complexed, CLC signals through a tripartite receptor complex composed of gp130, LIF R beta and CNTF R alpha (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8). Human to mouse, mature CLC shares 96% aa sequence identity. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9 - 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).

1. Murakami, M. et al. (2004) Growth Factors 22:75.
2. Ernst, M. and B.J. Jenkins (2004) Trends Genet. 20:23.
3. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
4. Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
5. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
6. Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
8. Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
9. Forger, N.G. et al. (2003) J. Neurosci. 23:8854
10. Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
11. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
12. de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.

Bioinformatics

• Uniprot:
  • Q9UBD9 (CLC) & P26992 (CNTF R alpha )()