Recombinant Human CL-P1/COLEC12 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human CL-P1/COLEC12 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rhCL-P1 at 5 µg/mL (100 µL/well) can bind biotinylated advance glycation endproducts of bovine serum albumin (AGE-BSA) with a linear range of
  3-200 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human CL-P1/COLEC12 protein
  Leu57-Leu742, with an N-terminal 9-His tag
• Accession #: EAX01728
• N-terminal Sequence: His
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: COLEC12
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 76 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 125-130 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CL-P1/COLEC12 Protein, CF

• CL-P1
• CLP1hCL-P1
• COLEC12
• collectin placenta 1
• Collectin placenta protein 1
• collectin sub-family member 12
• collectin-12
• NSR2
• Nurse cell scavenger receptor 2
• SCARA4
• SCARA4NSR2
• Scavenger receptor class A member 4
• scavenger receptor class A, member 4
• SRCL Type I
• SRCLScavenger receptor with C-type lectin

Background

Collectins are a family of Ca⁺⁺-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1 - 4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). With two exceptions, all collectins are secreted. CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo-orosomucoids associated with the Lewis^x epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full-length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical within each species CRD (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5). CL-P1 may play a role in bacterial recognition or as a scavenger receptor for desialylated.

1. van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
5. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
6. Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
7. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
8. Yoshida, T. et al. (2003) J. Biochem. 133:271.
9. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.

Publications for CL-P1/COLEC12 (2690-CL)(2)

Publications using 2690-CL

• J Zhang, A Li, CQ Yang, P Garred, YJ Ma Rapid and Efficient Purification of Functional Collectin-12 and Its Opsonic Activity against Fungal Pathogens J Immunol Res, 2019-07-29;2019(0):9164202. 2019-07-29 [PMID: 31482100] (ELISA Standard, Western Blot Control, Human)
• Ma Y, Hein E, Munthe-Fog L, Skjoedt M, Bayarri-Olmos R, Romani L, Garred P Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway. J Immunol, 2015-08-19;195(7):3365-73. 2015-08-19 [PMID: 26290605] (ELISA (Standard), Human)

Bioinformatics

• Gene Symbol: COLEC12
• Uniprot:
  • EAX01728()