Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF

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2 μg/lane of Recombinant Human CEACAM‑3 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 48-54 kDa and 96-110 kDa, ...read more
When Recombinant Human CEACAM-3/CD66d Fc Chimera (Catalog #9985-CM) is immobilized at 1 µg/mL, Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED50 of 0.05‑0.4 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human CEACAM‑3/CD66d Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Human CEACAM-7 (Catalog # 9010-CM) binds with an ED50 of 0.05-0.4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CEACAM-3/CD66d protein
Human CEACAM-3
(Lys35-Gly155)
Accession # P40198
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Lys35
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
39.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
48-54 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CEACAM-3/CD66d Fc Chimera Protein, CF

  • CD66d
  • CEA
  • CEACAM3 carcinoembryonic antigen-related cell adhesion molecule 3
  • CEACAM-3
  • CGM1
  • W264
  • W282

Background

Carcinoembryonic Antigen-related Cell Adhesion Molecule 3 (CEACAM-3), or CD66d, is part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). Both CEACAM and PSG molecules have been identified in humans and belong to the much larger glycosylphosphatidylinositol (GPI)-linked immunoglobulin (Ig) superfamily (1, 2). Human CEACAM-3 is ~35 kDa, consisting of an extracellular domain (ECD) containing one IgV-like domain, a single transmembrane domain and a cytoplasmic tail. The cytoplasmic tail of CEACAM-3 contains an immunoreceptor tyrosine-based activation motif (ITAM), which recruits kinases to propagate pro-inflammatory signaling cascades (3). Interestingly, CEACAM-3 appears to be primate specific, with on non-primate orthologs currently identified (4). Originally discovered as a biomarker for colorectal cancer (5), CEACAMs have now been associated with numerous intracellular signaling processes including cell adhesion, cell growth, recognition and differentiation, angiogenesis, and apoptosis (6-8). Unlike other CEA family members, CEACAM-3 has not been shown to form cell–cell adhesion interactions with other CEACAM family members (9). CEACAM-3 has been found to be specifically expressed on human neutrophils and other granulocytes and appears to be a specific adaptation of the innate immune system to cope with a small set of host-specific pathogen (9). CEACAM-3 was identified as critical for opsonin-independent phagocytosis and bacterial clearance (10). CEACAM-3 binds to the colony opacity-associated (Opa) outer membrane proteins of bacteria, such as Neisseria gonorrhoeae, and triggers uptake of the pathogen and subsequent elimination (9, 10).
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Zebhauser R, et al. (2005) Genomics 86:566.
  3. Schmitter, T. et al. (2004) J. Exp. Med. 199:35.
  4. Pavlopoulou A. and Scorilas A. (2014) Genome Biol Evol. 6:1314.
  5. Gold P and Freedman, S.O. (1965) J Exp Med. 122:467.
  6. Obrink, B. (1997) Curr Opin Cell Biol. 9:616.
  7. Horst, AK. and Wagener, C. (2004) Handb Exp Pharmacol. 165:283.
  8. Kuespert K et al. (2006) Curr Opin Cell Biol. 18:565.
  9. Pils S. et al. (2008) Int J Med Microbiol. 298:553.
  10. Schmitter, T. et al. (2004) J. Exp. Med. 199:35.

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