Recombinant Human CD46 His-tag Protein, CF

When Recombinant Human CD46 (Catalog # 10256-CD) is immobilized at 5 μg/mL (100 μL/well), it binds to Human C4b with an ED50 of 2‑12 μg/mL.

2 μg/lane of Recombinant Human CD46 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-67 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD46 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human CD46 is immobilized at 5 μg/mL (100 μL/well), the concentration Human C4b that produces 50% of the optimal binding response is 2-12 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human CD46 protein
  Cys35-Asp328, with a C-terminal 10-His tag
• Accession #: P15529-11
• N-terminal Sequence: Cys35
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-67 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD46 His-tag Protein, CF

• AHUS2
• CD46 antigen
• CD46 molecule, complement regulatory protein
• CD46
• MCP
• membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
• MIC10
• TLXcomplement regulatory protein
• trophoblast leucocyte common antigen
• Trophoblast leukocyte common antigen
• trophoblast-lymphocyte cross-reactive antigen

Background

CD46, also known as MCP (Membrane cofactor protein), is a type I membrane protein that functions as a cofactor for Complement Factor I­mediated inactivation of complement components C3b and C4b (1, 2). Mature human CD46 consists of a 309 amino acid (aa) extracellular domain (ECD) with four short consensus repeats (SCR) and a Ser/Thr/Pro-enriched region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain (1). Within the ECD, human CD46 shares 50% aa sequence identity with mouse and rat CD46. CD46 is commonly expressed in four distinct isoforms, and all isoforms have conserved complement control protein domains which harbor the C3b and C4b binding sites (3). CD46 is expressed on all nucleated cells (4). CD46 participates in the maintenance of immune homeostasis and the induction of protective T cell immunity, and it could extend towards key processes in development and cell metabolism (5).

1. Liszewski, M.K. et al. (1991) Annu. Rev. Immunol. 9:431.
2. Barilla-LaBarca, M.L. et al. (2002) J. Immunol. 168:6298.
3. Yamamoto, H. et al. (2013) Intl. J. Biochem. Cell Biol. 45:2808.
4. Andrews, P.W. et al. (1985) Ann. Hum. Genet. 49:31.
5. Kolev, M. et al. (2013) Semin. Immunol. 25:12.

Publications for CD46 (10256-CD)(1)

Publication using 10256-CD

• Kupke, P;Yang Zhou, J;Glehr, G;Riquelme, P;Scheibert, L;Adenugba, A;Schlitt, HJ;Geissler, EK;Werner, JM;Hutchinson, JA; Proteolytic shedding of CD46 from human hepatocytes indicates liver stress Heliyon 2024-12-15 [PMID: 39698100] (ELISA Standard, Human)

Bioinformatics

• Uniprot:
  • P15529-11()