Recombinant Human CD3 delta/CD3d Fc Avi-tag Protein, CF

When Recombinant Human CD3 epsilon Fc Chimera Protein (9850-CD) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL.

2 μg/lane of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-61 kDa and 108-122 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD3 delta/CD3d Fc Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD3 epsilon Fc Chimera Protein (Catalog # 9850-CD) is immobilized at 10 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human CD3 delta/CD3d Fc Chimera Avi-tag (Catalog # AVI10280) that produces 50% of the optimal binding response is approximately 2.0-14 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CD3 delta/CD3d protein
  

  Human CD3 delta/CD3d (Phe22-Ala105) Accession # P04234.1	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: P04234.1
• N-terminal Sequence: Phe22
• Structure / Form: Disulfide-linked homodimer, biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-61 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD3 delta/CD3d Fc Avi-tag Protein, CF

• CD3 antigen, delta subunit
• CD3 delta
• CD3d antigen
• CD3d antigen, delta polypeptide (TiT3 complex)
• CD3d molecule, delta (CD3-TCR complex)
• CD3D
• CD3-DELTA
• T3DOKT3, delta chain
• T-cell receptor T3 delta chain
• T-cell surface glycoprotein CD3 delta chain

Background

CD3d, also known as CD3 delta and T3D, is a single-pass type I membrane protein. CD3 delta/CD3d is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction (1). The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T cells (2). CD3 delta/CD3d contains an 84 amino acid extracellular domain, a 21 amino acid transmembrane domain, and a 45 amino acid cytoplasmic domain. Human CD3 delta/CD3d shares 89%, 61%, and 57% sequence identity in its extracellular domain with its cynomolgus monkey, rat, and mouse homologs, respectively. CD3 delta/CD3d, like other subunit members CD3G and CD3E of the TCR-CD3 complex, contains an extracellular Ig domain and a single immunoreceptor tyrosine-based activation motif (3, 4). Defects in CD3 delta/CD3d cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels (5, 6). Our Avi-tag Biotinylated human CD-3D Fc Chimera protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Gil, J. et al. (2011) J. Clin. Invest. 121:3872.
2. Carson, G.R. et al. (1991) J. Biol. Chem. 266:7883.
3. Birnbaum, M.E. et al. (2014). Proc. Natl. Acad. Sci. USA. 111:17576.
4. Wucherpfennig, K. et al. (2010). Cold Spring Harb Perspect. Biol. 2:a005140.
5. Kim, S.T. et al. (2010) J. Immunol. 185:2951.
6. Holst, J. et al. (2008) Nat. Immunol. 9:658.

Bioinformatics

• Uniprot:
  • P04234.1()