Recombinant Human CD200 His-tag Avi-tag Protein, CF

When Recombinant Human CD200R1 Fc Chimera (3414-CD) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD200 His-tag Avi-tag (Catalog # AVI10032) binds with an ED50 of 0.1-0.6 µg/mL.

2 μg/lane of Biotinylated Recombinant CD200 His-tag Avi-tag Protein (Catalog # AVI10032) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-50 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CD200 His-tag Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD200R1 Fc Chimera (Catalog # 3414-CD) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human CD200 His-tag Avi-tag (Catalog # AVI10032) binds with an ED₅₀ of 0.1-0.6 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human CD200 protein
  

  Human CD200 (Gln31-Lys232) Accession # AAH22522.1	HHHHHH	Avi-tag
  N-terminus		C-terminus

• Accession #: AAH22522.1
• N-terminal Sequence: Gln31 inferred from enzymatic pyroglutamate treatment revealing Val32.
• Structure / Form: Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-50 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD200 His-tag Avi-tag Protein, CF

• antigen identified by monoclonal MRC OX-2
• CD200 antigenMOX1
• CD200 molecule
• CD200
• MOX1
• MOX2
• MOX2MRC
• MRC OX-2 antigen
• MRC
• OX-2 membrane glycoprotein
• OX-2

Background

Cluster of Differentiation-200 (CD200), also known as OX-2, is a transmembrane immunoregulatory protein that belongs to the immunoglobulin superfamily (1, 2). Mature human CD200 consists of an extracellular domain (ECD) with one Ig-like V-type domain and one Ig-like C2-type domain, a transmembrane segment, and a short cytoplasmic domain (3). Within the mature ECD, human CD200 shares 76% amin acid (aa) sequence identity with mouse and rat CD200. A splice variant of CD200 has been identified lacking the first 43 aa of the ECD and characterized as a functional antagonist to full-length CD200 (4). CD200 is widely expressed by numerous cell types including neurons, B cells, activated T cells, thymocytes, dendritic cells and endothelium (5). Its receptor, CD200R, is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests CD200 is an important inhibitory ligand for myeloid cell regulation (6-8). CD200 knockout mice are characterized by increased macrophage number and activation and are predisposed to autoimmune disorders (9). CD200 and CD200R associate via their respective N-terminal Ig-like domains (10). In myeloid cells, CD200R initiates inhibitory signals following receptor-ligand contact (7, 8, 11). In T cells, however, CD200 functions as a costimulatory molecule independent of the CD28 pathway (12). Several additional CD200R-like molecules have been identified in human and mouse, but their capacity to interact with CD200 is controversial (13, 14). Several viruses encode CD200 homologs which are expressed on infected cells during the lytic phase (15, 16). Like CD200 itself, viral CD200 homologs also suppress myeloid cell activity, enabling increased viral propagation (6, 15-17). Our Avi-tag Biotinylated human CD200 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Gorczynski, R.M. (2005) Curr. Opin. Invest. Drugs 6:483.
2. Barclay, A.N. et al. (2002) Trends Immunol. 23:285.
3. McCaughan, G.W. et al. (1987) Immunogenetics 25:329.
4. Chen, Z. et al. (2008) Transplantation. 86:1116
5. Wright, G.J. et al. (2001) Immunology 102:173.
6. Shiratori, I. et al. (2005) J. Immunol. 175:4441.
7. Cherwinski, H.M. et al. (2005) J. Immunol. 174:1348.
8. Fallarino, F. et al. (2004) J. Immunol. 173:3748.
9. Hoek, R.M. et al. (2000) Science 290:1768.
10. Hatherley, D. and A.N. Barclay (2004) Eur. J. Immunol. 34:1688.
11. Jenmalm, M.C. et al. (2006) J. Immunol. 176:191.
12. Borriello, F. et al. (1997) J. Immunol. 158:4548.
13. Gorczynski, R. et al. (2004) J. Immunol. 172:7744.
14. Hatherley, D. et al. (2005) J. Immunol. 175:2469.
15. Foster-Cuevas, M. et al. (2004) J. Virol. 78:7667.
16. Cameron, C.M. et al. (2005) J. Virol. 79:6052.
17. Langlais, C.L. et al. (2006) J. Virol. 80:3098.

Bioinformatics

• Uniprot:
  • AAH22522.1()