Recombinant Human CCL28 Protein

Recombinant Human CCL28 (Catalog # 717‑VC) chemoattracts the BaF3 mouse pro-B cells transfected with mouse CCR10. The ED50 for this effect is 0.4‑2.0 μg/mL.

1 μg/lane of Recombinant Human CCL28 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human CCL28 Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR10. The ED₅₀ for this effect is 0.4-2.0 µg/mL.
• Source: E. coli-derived human CCL28 protein
  Ile23-Tyr127
• Accession #: Q9NRJ3
• N-terminal Sequence: Ile23
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL28
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL28 Protein

• CCL28
• chemokine (C-C motif) ligand 28
• MEC
• member 28
• MGC71902
• Protein CCK1
• VIC

Background

Human CCL28 (CC chemokine ligand 28) is a novel CC chemokine identified by TBLASTN searches of the Human Genome Systems (HGS) and Genbank dbEst database using a human chemokine consensus sequence. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 are highly conserved, sharing 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. The mouse CCL28 gene has been mapped to the distal region of chromosome 13. Human and mouse CCL28 RNA expression was found to be highest in normal and pathologic colon with the protein being expressed by epithelial cells. Human CCL28 RNA was also present in normal and asthmatic lung tissues. The receptor for CCL28 has been identified as the CCR10 (GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.

1. Wang, W. et al. (2000) J. Biol. Chem. 275:22313.

Publications for CCL28 (717-VC)(8)

Publications using 717-VC

• M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals Mucosal Immunol, 2020-04-28;0(0):. 2020-04-28 [PMID: 32346082] (Bioassay, Human)
• T Karnezis, R Farnsworth, NC Harris, SP Williams, C Caesar, DJ Byrne, P Herle, ML Macheda, R Shayan, YF Zhang, S Yazar, SJ Takouridis, C Gerard, S Fox, MG Achen, SA Stacker CCL27/CCL28-CCR10 CHEMOKINE SIGNALING MEDIATES MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS Cancer Res., 2019-02-01;0(0):. 2019-02-01 [PMID: 30709930] (Bioassay, In Vivo, Human, Mouse)
• Guichun Huang Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells Sci Rep, 2016-06-02;6(0):27152. 2016-06-02 [PMID: 27250766] (Bioassay, Human)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Pacheco-Rodriguez G, Kumaki F, Steagall WK, Zhang Y, Ikeda Y, Lin JP, Billings EM, Moss J Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene. J. Immunol., 2009-02-01;182(3):1270-7. 2009-02-01 [PMID: 19155472] (Bioassay, Human)
• Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006-04-17;350(2):484-92. 2006-04-17 [PMID: 16603217] (Binding Assay, Bioassay, Virus)
• Hieshima K, Ohtani H, Shibano M, Izawa D, Nakayama T, Kawasaki Y, Shiba F, Shiota M, Katou F, Saito T, Yoshie O CCL28 has dual roles in mucosal immunity as a chemokine with broad-spectrum antimicrobial activity. J. Immunol., 2003-02-01;170(3):1452-61. 2003-02-01 [PMID: 12538707] (Bioassay, ELISA (Standard), Western Blot, Human)
• Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002-06-15;168(12):6244-52. 2002-06-15 [PMID: 12055238] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CCL28
• Uniprot:
  • Q9NRJ3()