Recombinant Human CCL22/MDC Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CCL22/MDC Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CCR4. The ED 50 for this effect is 0.5-3 ng/mL.
• Source: E. coli-derived human CCL22/MDC protein Gly25-Gln93
• Accession #: O00626.1
• N-terminal Sequence: Gly25
• Protein/Peptide Type: Recombinant Proteins
• Gene: CCL22
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CCL22/MDC Protein, CF

• A-152E5.1
• ABCD-1
• CC chemokine STCP-1
• C-C motif chemokine 22
• CCL22
• chemokine (C-C motif) ligand 22
• DC/B-CK
• Macrophage-derived chemokine
• MDC
• MDCStimulated T-cell chemotactic protein 1
• MGC34554
• SCYA22MDC(1-69)
• small inducible cytokine A22
• small inducible cytokine subfamily A (Cys-Cys), member 22
• Small-inducible cytokine A22
• STCP-1
• stimulated T cell chemotactic protein 1

Background

CCL22, also named stimulated T cell chemotactic protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca 2+ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8 + T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T-cell line-adapted isolate HIV-1 IIIB has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8 + T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.

1. Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
2. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.
3. Pal, R. et al. (1997) Science 278:5338.

Publications for CCL22/MDC (336-MD/CF)(25)

Publications using 336-MD/CF

• Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells. iScience, 2021-11-14;24(12):103433. 2021-11-14 [PMID: 34917892] (Bioassay, Human)
• K Jamal Jame, WJ Gallert, SD Yanik, S Panek, J Kronsbein, D Jungck, A Koch, J Knobloch Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD International Journal of Molecular Sciences, 2021-07-02;22(13):. 2021-07-02 [PMID: 34281240] (Bioassay, Human)
• C Kötting, L Hofmann, R Lotfi, D Engelhardt, S Laban, PJ Schuler, TK Hoffmann, C Brunner, MN Theodoraki Immune-Stimulatory Effects of Curcumin on the Tumor Microenvironment in Head and Neck Squamous Cell Carcinoma Cancers, 2021-03-16;13(6):. 2021-03-16 [PMID: 33809574] (Human)
• J Okano, Y Nakae, T Nakagawa, M Katagi, T Terashima, D Nagakubo, T Nakayama, O Yoshie, Y Suzuki, H Kojima A novel role for bone marrow-derived cells to recover damaged keratinocytes from radiation-induced injury Scientific Reports, 2021-03-11;11(1):5653. 2021-03-11 [PMID: 33707490] (Bioassay, Human)
• JD Fisher, W Zhang, SC Balmert, AM Aral, AP Acharya, Y Kulahci, J Li, HR Turnquist, AW Thomson, MG Solari, VS Gorantla, SR Little In situ recruitment of regulatory T cells promotes donor-specific tolerance in vascularized composite allotransplantation Sci Adv, 2020-03-13;6(11):eaax8429. 2020-03-13 [PMID: 32201714] (Cell Culture, Human)
• Anti-Inflammatory Effects of Metformin Irrespective of Diabetes Status Circ Res, 2016-07-14;0(0):. 2016-07-14 [PMID: 27418629] (Bioassay, Human)
• Yu , Qing, Lou , Xiang-mi, He , Yan Preferential recruitment of Th17 cells to cervical cancer via CCR6-CCL20 pathway. PLoS ONE, 2015-03-13;10(3):e0120855. 2015-03-13 [PMID: 25768730] (Bioassay, Human)
• Nakagawa , Masao, Schmitz , Roland, Xiao , Wenming, Goldman , Carolyn, Xu , Weihong, Yang , Yandan, Yu , Xin, Waldmann , Thomas A, Staudt , Louis M Gain-of-function CCR4 mutations in adult T cell leukemia/lymphoma. J Exp Med, 2014-12-08;211(13):2497-505. 2014-12-08 [PMID: 25488980] (Bioassay, Mouse)
• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Bioassay, Human)
• Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation. Blood, 2008-05-14;112(3):493-503. 2008-05-14 [PMID: 18480427] (Bioassay, Human)
• Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis. Blood, 2007-10-29;111(2):761-6. 2007-10-29 [PMID: 17967942] (Bioassay, Human)
• Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha. J. Immunol., 2007-10-01;179(7):4840-8. 2007-10-01 [PMID: 17878383] (ELISA (Standard))
• Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. J. Immunol., 2006-10-01;177(7):4488-94. 2006-10-01 [PMID: 16982885] (Bioassay, Human)
• Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O Novel antiviral activity of chemokines. Virology, 2006-04-17;350(2):484-92. 2006-04-17 [PMID: 16603217] (Binding Assay, Bioassay, Virus)
• Gunther C, Bello-Fernandez C, Kopp T, Kund J, Carballido-Perrig N, Hinteregger S, Fassl S, Schwarzler C, Lametschwandtner G, Stingl G, Biedermann T, Carballido JM CCL18 is expressed in atopic dermatitis and mediates skin homing of human memory T cells. J. Immunol., 2005-02-01;174(3):1723-8. 2005-02-01 [PMID: 15661937] (In Vivo, Mouse)
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-12-01;173(11):6592-602. 2004-12-01 [PMID: 15557149] (Bioassay, Human)
• Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M TARC augments TNF-alpha-induced CTACK production in keratinocytes. Exp. Dermatol., 2004-09-01;13(9):551-7. 2004-09-01 [PMID: 15335355] (Bioassay, Human)
• Curiel , Tyler J, Coukos , George, Zou , Linhua, Alvarez , Xavier, Cheng , Pui, Mottram , Peter, Evdemon-Hogan , Melina, Conejo-Garcia , Jose R, Zhang , Lin, Burow , Matthew, Zhu , Yun, Wei , Shuang, Kryczek , Ilona, Daniel , Ben, Gordon , Alan, Myers , Leann, Lackner , Andrew, Disis , Mary L, Knutson , Keith L, Chen , Lieping, Zou , Weiping Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival. Nat Med, 2004-08-22;10(9):942-9. 2004-08-22 [PMID: 15322536] (Bioassay, In Vivo, Human, Xenograft)
• Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia. Am. J. Respir. Crit. Care Med., 2004-06-10;170(5):508-15. 2004-06-10 [PMID: 15191918] (Bioassay, Human)
• Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-06-01;112(2):274-89. 2004-06-01 [PMID: 15147571] (Bioassay, Human)
• Liu L, Jarjour NN, Busse WW, Kelly EA Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma. Am. J. Respir. Crit. Care Med., 2004-03-04;169(10):1118-24. 2004-03-04 [PMID: 15001464] (ELISA (Standard))
• Conroy DM, Jopling LA, Lloyd CM, Hodge MR, Andrew DP, Williams TJ, Pease JE, Sabroe I CCR4 blockade does not inhibit allergic airways inflammation. J. Leukoc. Biol., 2003-07-15;74(4):558-63. 2003-07-15 [PMID: 12960268] (ELISA (Standard))
• Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002-06-15;168(12):6244-52. 2002-06-15 [PMID: 12055238] (Bioassay, Human)
• de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo. Eur. J. Immunol., 2001-04-01;31(4):1037-46. 2001-04-01 [PMID: 11298328] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CCL22
• Uniprot:
  • O00626.1()