Recombinant Human Carbonic Anhydrase X/CA10 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Carbonic Anhydrase X/CA10 Protein, CF Summary

• Source: Mouse myeloma cell line, NS0-derived human Carbonic Anhydrase X/CA10 protein
  Ala21-Asn300, with a C-terminal 10-His tag
• Accession #: Q9NS85
• N-terminal Sequence: Ala21
• Protein/Peptide Type: Recombinant Enzymes
• Gene: CA10
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Carbonic Anhydrase X/CA10 Protein, CF

• CA10
• Carbonic Anhydrase X
• carbonic anhydrase-related protein 10
• Carbonic anhydrase-related protein X
• CARP X
• CARPX
• CA-RPX
• CARPXCA-RP X
• Cerebral protein 15
• cerebral protein-15
• HUCEP-15

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA10, also known as CA-related protein, CA-RA X, is a secreted protein without CA activity (i.e., the reversible hydration of CO₂) due to point mutations in the zinc-binding site. The human and mouse CA10 are expressed in the brain, indicating that they may play a role in brain development (2, 3).

1. Hewett-Emmett, D. and R.E Tashian  (1996) Mol. Phylogenet. Evol. 5:50. 
2. Okamoto, N. et al. (2001) Biochim. Biophys. Acta 1518:311.
3. Taniuchi, K. et al. (2002) Brain Res. Mol. Brain Res. 109:207.

Bioinformatics

• Gene Symbol: CA10
• Uniprot:
  • Q9NS85()