Recombinant Human BMPR-IB/ALK-6 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human BMPR-IB/ALK-6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED₅₀ for this effect is 0.5‑1.5 µg/mL in the presence of 30 ng/mL of rhBMP-4.
• Source: Mouse myeloma cell line, NS0-derived human BMPR-IB/ALK-6 protein
  

  Met	Human BMPR-IB (Lys14 - Arg126) Accession # O00238	IEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus				C-terminus

• Accession #: O00238
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: BMPR1B
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMPR-IB/ALK-6 Fc Chimera Protein, CF

• activin receptor-like kinase 6
• ALK-6
• ALK6CDw293
• BMP type-1B receptor
• BMPR1B
• BMPR-1B
• BMPRIB
• BMPR-IB
• bone morphogenetic protein receptor type-1B
• bone morphogenetic protein receptor, type IB
• CDw293 antigen
• CDw293
• EC 2.7.11
• EC 2.7.11.30
• serine/threonine receptor kinase

Background

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is a one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% sequence identity.

1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.

Publications for BMPR-IB/ALK-6 (505-PR)(8)

Publications using 505-PR

• B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface Sci Rep, 2016-04-14;6(0):24323. 2016-04-14 [PMID: 27075233] (Bioassay, Mouse)
• Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth. J. Biol. Chem., 2011-07-07;286(34):30034-46. 2011-07-07 [PMID: 21737454] (Surface Plasmon Resonance, Human)
• Ambrosio AL, Taelman VF, Lee HX, Metzinger CA, Coffinier C, De Robertis EM Crossveinless-2 Is a BMP feedback inhibitor that binds Chordin/BMP to regulate Xenopus embryonic patterning. Dev. Cell, 2008-08-01;15(2):248-60. 2008-08-01 [PMID: 18694564] (Binding Assay, Human)
• Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling. Blood, 2008-07-29;112(8):3154-63. 2008-07-29 [PMID: 18664625] (Bioassay, Human)
• Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. J. Mol. Biol., 2008-07-02;381(4):1025-39. 2008-07-02 [PMID: 18621057] (ELISA (detection), Surface Plasmon Resonance, Human)
• Serpe M, Umulis D, Ralston A, Chen J, Olson DJ, Avanesov A, Othmer H, O'Connor MB, Blair SS The BMP-binding protein Crossveinless 2 is a short-range, concentration-dependent, biphasic modulator of BMP signaling in Drosophila. Dev. Cell, 2008-06-01;14(6):940-53. 2008-06-01 [PMID: 18539121] (Binding Assay, Drosophila)
• Reversade B, De Robertis EM Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field. Cell, 2005-12-16;123(6):1147-60. 2005-12-16 [PMID: 16360041] (Receptor Binding Assay, Human)
• Gunhaga L, Marklund M, Sjodal M, Hsieh JC, Jessell TM, Edlund T Specification of dorsal telencephalic character by sequential Wnt and FGF signaling. Nat. Neurosci., 2003-07-01;6(7):701-7. 2003-07-01 [PMID: 12766771] (Bioassay, Chicken)

Bioinformatics

• Gene Symbol: BMPR1B
• Uniprot:
  • O00238()