Reactivity | HuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Additional Information | Biotinylated long-form B7-H3 (4Ig)/B7-H3b |
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Details of Functionality | Measured in a cell proliferation assay using mouse CD4+ cells in the presence of anti-CD3. The ED50 for this effect is 3-12 μg/mL. Measured by its binding ability in a functional ELISA. When Human B7-H3 Antibody
(Catalog #
AF1027)
is immobilized
at 1.0 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human B7‑H3 His-tag Avi-tag (Catalog # AVI2318) that produces 50% of
the optimal binding response is approximately 3 -15 ng/mL. |
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Source | Human embryonic kidney cell, HEK293-derived human B7-H3 protein
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Accession # | |||||||
N-terminal Sequence | Leu29 |
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Structure / Form | Biotinylated via Avi-tag |
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Protein/Peptide Type | Recombinant Proteins |
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Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
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Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 49 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE | 70-90 kDa, under reducing conditions |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 200 μg/mL in PBS. |
Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses (1 - 3). Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V‑type and one C‑type Ig domain, a transmembrane segment and a short cytoplasmic tail (1). Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern (4, 5). It would appear that the human 4Ig form is the principal, if not the only form of B7-H3 (5). Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V‑type and C‑type, and apparently are the consequence of a V‑C type tandem duplication (4, 5). B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells (5). The mouse gene differs from that of human in that it cannot code for four Ig-like domains; only a V‑type:C‑type pair (4). Human B7-H3b binding to an undefined receptor has shown to be inhibitory to NK cells and cytokine release (6). It also seems to be required for late stage osteoblast differentiation (7).
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