Recombinant Human ASP/C3a desArg Protein, CF

Recombinant Human ASP/C3a desArg (Catalog # 9620-C3/CF) induces IL-6 secretion from LPS stimulated PBMCs. The ED50 for this effect is 0.4-2.4 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ASP/C3a desArg Protein, CF Summary

• Details of Functionality: Measured by its ability to induce IL-6 secretion by LPS-stimulated human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 0.4-2.4 μg/mL.
• Source: E. coli-derived human ASP/C3a desArg protein
  Ser672-Ala747
• Accession #: P01024
• N-terminal Sequence: Ser672
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 9 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ASP/C3a desArg Protein, CF

• Acylation Stimulating Protein
• ASP
• C3a des Arg

Background

Acylation-stimulating protein (ASP), also known as C3adesArg, is an approximately 14 kDa member of the complement family that plays a role in the regulation of triglyceride synthesis (1-4). The secreted protein is a polypeptide fragment comprising amino acids (aa) 672-747 of the Complement C3 precursor protein (4). The polypeptide results from desargination of the carboxyl terminus of C3a, which is generated from interactions among C3, factor B, and adipsin (4). ASP contains a 36 aa anaphylatoxin-like domain (5). Human ASP shares 68% sequence identity with mouse and rat ASP. Increased levels of ASP are usually associated with insulin resistance, obesity and diabetes. It promotes triacylglycerol synthesis in adipocytes and inflammatory cytokine production in a septic environment (6-8).

1. Cianflone, K. et al. (1989) J. Biol. Chem. 264:426.
2. Baldo, A. et al. (1993) J. Clin. Invest. 92:1543.
3. Havel, P. (2002) Curr. Opin. Lipidol. 13:51.
4. Cianflone, K. et al. (2003) Biochim. Biophys. Acta. 1609:127.
5. Kolev, M. et al. (2017) Front. Immunol. 8:1.
6. Murray, I. et al. (1999) Biochem. J. 342:41.
7. Munkonda, M. N. et al. (2012) PLOS One. 7(10):1.
8. Takabayashi, T. et al. (1998) JID. 177:1622.

Bioinformatics

• Uniprot:
  • P01024()