Recombinant Human Annexin A13 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Annexin A13 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: E. coli-derived human Annexin A13 protein
  Gly2-His316 (Arg86His), with a C-terminal 6-His tag
• Accession #: P27216-1
• N-terminal Sequence: Gly2
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Annexin A13 Protein, CF

• Annexin A13
• Annexin XIII
• Annexin-13
• ANX13
• ANX13annexin, intestine-specific
• ANXA13
• Intestine-specific annexin
• ISA
• ISAannexin-13
• MGC150460

Background

Human Annexin A13 (ANXA13), also known as Annexin XIII or Annexin 13, is a 35 kDa member of the Annexin family (1), which are calcium-dependent phospholipid-binding proteins being preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain of Annexin A13 is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the Annexin domain. Alternate splicing generates an additional isoform with a 41 amino acid (aa) deletion near the N‑terminus (2). Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (3, 4). Human Annexin A13 shares approximately 86% and 91% aa sequence identity with mouse and dog Annexin A13 respectively.

1. Wice, B. et al. (1992) J Cell Biol. 116:405.
2. Turnay, J. et al. (2005) Biochem. J. 389:899.
3. Gerke, V. et al. (2002) Physiol. Rev. 82:331.
4. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.

Bioinformatics

• Uniprot:
  • P27216-1()