Recombinant Human Annexin A10 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Annexin A10 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: E. coli-derived human Annexin A10 protein
  Met1-Tyr324, with a C-terminal 6-His tag
• Accession #: Q9UJ72
• N-terminal Sequence: Met1
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Annexin A10 Protein, CF

• annexin 14
• Annexin A10
• annexin-10
• annexin-14
• ANX14
• ANX14Annexin-10
• ANXA10

Background

Human Annexin A10 (ANXA10), also known as Annexin 10 or Annexin-14, is a 37 kDa member of the Annexin family (1), which are calcium-dependent phospholipid-binding proteins preferentially being located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (2). Human Annexin A10 shares approximately 89% and 88% aa sequence identity with mouse and rat Annexin A10 respectively.

1. Morgan, R. et al. (1999) Genomics 60:40.
2. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.

Bioinformatics

• Uniprot:
  • Q9UJ72()