Recombinant Human Angiopoietin-like 7 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Angiopoietin-like 7 Protein, CF Summary

• Details of Functionality: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED₅₀ for this effect is 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
• Source: Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 7/ANGPTL7 protein
  Met1-Pro346, with a C-terminal 10-His tag
• Accession #: O43827
• N-terminal Sequence: No results obtained: Gln27 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: ANGPTL7
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 38.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 49-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like 7 Protein, CF

• angiopoietin-like 7
• Angiopoietin-like factor
• Angiopoietin-like Protein 7
• angiopoietin-related protein 7
• ANGPTL7
• AngX
• CDT6
• CDT6angiopoietin-like factor (CDT6)
• Cornea-derived transcript 6 protein
• dJ647M16.1
• RP4-647M16.2

Background

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1 - 3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45 ‑ 50 kDa monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up‑regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5 - 7). Overexpression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When overexpressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells (9).

1. Oike, Y. et al. (2009) Circ. J. 73:2192.
2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
3. Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782.
4. Peek, R. et al. (2002) J. Biol. Chem. 277:686.
5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438.
6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
8. Comes, N. et al. (2010) Genes Cells 16:243.
9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Publications for Angiopoietin-like Protein 7/ANGPTL7 (914-AN/CF)(2)

Publications using 914-AN/CF

• Obenauf A, Zou Y, Ji A, Vanharanta S, Shu W, Shi H, Kong X, Bosenberg M, Wiesner T, Rosen N, Lo R, Massague J Therapy-induced tumour secretomes promote resistance and tumour progression. Nature, 2015-03-25;520(7547):368-72. 2015-03-25 [PMID: 25807485] (Bioassay, Human)
• Toyono , Tetsuya, Usui , Tomohiko, Yokoo , Seiichi, Taketani , Yukako, Nakagawa , Suguru, Kuroda , Masahiko, Yamagami , Satoru, Amano , Shiro Angiopoietin-like 7 is an anti-angiogenic protein required to prevent vascularization of the cornea. PLoS ONE, 2015-01-26;10(1):e0116838. 2015-01-26 [PMID: 25622036] (Bioassay, Human)

Bioinformatics

• Gene Symbol: ANGPTL7
• Uniprot:
  • O43827()