Recombinant Human Angiopoietin-like 7 Biotin Protein, CF

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Both Biotinylated rhANGPTL7 (Catalog # BT914) and unlabeled Recombinant Human Angiopoietin-likeProtein 7/ANGPTL7 (Catalog # 914-AN) stimulate the expansion of E16-E18 rat liver mononuclear cells in vitro, in the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Angiopoietin-like 7 Biotin Protein, CF Summary

Details of Functionality
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 7/ANGPTL7 protein
Gln27-Pro346, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
No results: Gln27 predicted, sequencing might be blocked
Structure / Form
Oligomer, Biotinylated on protein carbohydrates
Protein/Peptide Type
Recombinant Proteins
Gene
ANGPTL7
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
39 kDa (unlabeled).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
>110 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like 7 Biotin Protein, CF

  • angiopoietin-like 7
  • Angiopoietin-like factor
  • Angiopoietin-like Protein 7
  • angiopoietin-related protein 7
  • ANGPTL7
  • AngX
  • CDT6
  • CDT6angiopoietin-like factor (CDT6)
  • Cornea-derived transcript 6 protein
  • dJ647M16.1
  • RP4-647M16.2

Background

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1-3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45-50 kDa monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up-regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5-7). Over-expression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When over-expressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). ANGPTL7, when used in combination with SCF, Thrombopoietin, IGF-II, and FGF acidic, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (9).
  1. Oike, Y. et al. (2009) Circ. J. 73:2192.
  2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
  3. Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782.
  4. Peek, R. et al. (2002) J. Biol. Chem. 277:686.
  5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438.
  6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
  7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
  8. Comes, N. et al. (2010) Genes Cells 16:243.
  9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

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Bioinformatics

Gene Symbol ANGPTL7
Entrez
Uniprot