Recombinant Human Angiopoietin-like 7 Biotin Protein, CF

Both Biotinylated rhANGPTL7 (Catalog # BT914) and unlabeled Recombinant Human Angiopoietin-likeProtein 7/ANGPTL7 (Catalog # 914-AN) stimulate the expansion of E16-E18 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) , and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is 50-200 ng/mL in the presence of a cross-linking antibody, His Tag MAb (Catalog # MAB050) . The similarity in activity highlights that the biotinylated protein is fully functional.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Angiopoietin-like 7 Biotin Protein, CF Summary

• Details of Functionality: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED₅₀ for this effect is 50-200 ng/mL in the presence of a cross‑linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
• Source: Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 7/ANGPTL7 protein
  Gln27-Pro346, with a C-terminal 10-His tag
• Accession #: O43827
• N-terminal Sequence: No results: Gln27 predicted, sequencing might be blocked
• Structure / Form: Oligomer, Biotinylated on protein carbohydrates
• Protein/Peptide Type: Recombinant Proteins
• Gene: ANGPTL7
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39 kDa (unlabeled).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: >110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like 7 Biotin Protein, CF

• angiopoietin-like 7
• Angiopoietin-like factor
• Angiopoietin-like Protein 7
• angiopoietin-related protein 7
• ANGPTL7
• AngX
• CDT6
• CDT6angiopoietin-like factor (CDT6)
• Cornea-derived transcript 6 protein
• dJ647M16.1
• RP4-647M16.2

Background

Angiopoietin-like 7 (ANGPTL7), also known as Corneal-Derived Transcript 6 (CDT6), is a secreted glycoprotein that is structurally related to the angiopoietins. Members of this protein family contain an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain (1-3). ANGPTL7 shares 89% aa sequence identity with mouse and rat ANGPTL7. It is secreted as a 45-50 kDa monomer that forms disulfide-linked homotrimers and tetramers via the coiled coil domain (4, 5). ANGPTL7 is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor (3, 5). Its production is up-regulated in trabecular meshwork cells by glucocorticoids and TGF-beta and in cartilage by TNF-alpha (5-7). Over-expression of ANGPTL7 in trabecular meshwork cells inhibits the production of collagen and proteoglycans (8). When over-expressed in tumor cells it promotes collagen and proteoglycan deposition but inhibits tumor xenograft progression and tumor angiogenesis (4). ANGPTL7, when used in combination with SCF, Thrombopoietin, IGF-II, and FGF acidic, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (9).

1. Oike, Y. et al. (2009) Circ. J. 73:2192.
2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
3. Peek, R. et al. (1998) Invest. Opthalmol. Visual Sci. 39:1782.
4. Peek, R. et al. (2002) J. Biol. Chem. 277:686.
5. Kuchtey, J. et al. (2008) Invest. Opthalmol. Visual Sci. 49:3438.
6. Nehme, A. et al. (2009) BMC Med. Genomics 2:58.
7. Stevens, A.L. et al. (2008) Arthritis Rheum. 58:489.
8. Comes, N. et al. (2010) Genes Cells 16:243.
9. Zhang, C.C. et al. (2006) Nat. Med. 12:240.

Bioinformatics

• Gene Symbol: ANGPTL7
• Uniprot:
  • O43827()