Recombinant Human ADAM22 Protein, CF

Images

 
There are currently no images for ADAM22 (4908-AD).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human ADAM22 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is 20-100 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human ADAM22 protein
Gly26-His528 (Leu78Val), with a C-terminal 8-His tag
Accession #
N-terminal Sequence
Gly26 & Asn233
Structure / Form
Mixture of pro and mature forms
Protein/Peptide Type
Recombinant Enzymes
Gene
ADAM22
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
58 kDa (pro) & 34 kDa (mature).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-45 kDa & 69-80 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ADAM22 Protein, CF

  • ADAM 22
  • ADAM metallopeptidase domain 22
  • ADAM22
  • MDC2
  • metalloproteinase-disintegrin ADAM22-3
  • metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2
  • MGC149832

Background

A disintegrin and metalloproteinase 22 (ADAM22), also known as MDC2, is an 85-90 kDa transmembrane member of the M12B family of peptidases (1). Human ADAM22 contains an N-terminal 197 amino acid (aa) cleavable proregion and a 514 aa extracellular domain (ECD). The ECD contains a nonfunctional metalloprotease domain, an integrin‑binding disintegrin domain, a cysteine‑rich region, and one EGF-like domain (2). The cytoplasmic portion contains two phosphorylation motifs which bind to 14-3-3 proteins to mediate cell adhesion (3, 4). Alternative splicing generates multiple isoforms with a variety of insertions, deletions, and substitutions in the cytoplasmic region (2, 5, 6). Within the peptidase-like and disintegrin domains, human ADAM22 shares 96% sequence identity with mouse and rat ADAM22. ADAM22 is expressed on neurons, astrocytes, and Schwann cells in the cerebral cortex, hippocampus, and cerebellum and also on some low-grade gliomas (5, 7-9). It can be up-regulated in endocrine therapy-resistant breast cancer, and it contributes to tumor cell migration (10). It mediates cell adhesion through binding to Integrins alpha 6, alpha 9, and beta 3 (7). ADAM22 is localized to post-synaptic densities (11). It binds the secreted protein LGI1 which functions as a bridge to link ADAM22 with pre-synaptically expressed ADAM23 (9). The interaction of LGI1 with ADAM22 promotes AMPA receptor expression in hippocampal neurons and strengthens excitatory synapses (9). In axonal juxtaparanodes, ADAM22 is a component of the voltage-gated Kv1 potassium channel that responds to LGI1 (12). It also colocalizes with Kv1 channels in basket cell interneuron terminals of the cerebellum (12). ADAM22 additionally binds to Schwann cell derived LGI4 and is required for LGI4 to promote axon myelination (8). Mice lacking ADAM22 expression exhibit hypomyelination and profound ataxia (13).
  1. Edwards, D.R. et al. (2008) Mol. Aspects Med. 29:258.
  2. Sagane, K. et al. (1998) Biochem. J. 334:93.
  3. Godde, N.J. et al. (2006) J. Cell Sci. 119:3296.
  4. Zhu, P. et al. (2005) Biochem. Biophys. Res. Commun. 331:938.
  5. Harada, T. et al. (2000) Jpn. J. Cancer Res. 91:1001.
  6. Godde, N.J. et al. (2007) Gene 403:80.
  7. D’Abaco, G.M. et al. (2006) Neurosurgery 58:179.
  8. Ozkaynak, E. et al. (2010) J. Neurosci. 30:3857.
  9. Fukata, Y. et al. (2006) Science 313:1792.
  10. McCartan, D. et al. (2012) Cancer Res. 72:220.
  11. Fukata, Y. et al. (2010) Proc. Natl. Acad. Sci. USA 107:3799.
  12. Ogawa, Y. et al. (2010) J. Neurosci. 30:1038.
  13. Sagane, K. et al. (2005) BMC Neurosci. 6:33.

Customers Who Viewed This Item Also Viewed...

MAB4974
Species: Hu
Applications: IHC
NBP1-77320
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
NBP2-67246
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-62689
Species: Hu
Applications: IHC, IHC-P
NBP2-56992
Species: Hu
Applications: IHC, IHC-P
NB100-395
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NBP1-88347
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF949
Species: Hu, Mu
Applications: WB, Flow, IHC, IP, CyTOF-ready
NBP2-30423
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-889
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
AF1376
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready
930-ADB
Species: Hu
MAB141
Species: Hu
Applications: WB, Flow, CyTOF-ready, KO, Neut
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P

Publications for ADAM22 (4908-AD) (0)

There are no publications for ADAM22 (4908-AD).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ADAM22 (4908-AD) (0)

There are no reviews for ADAM22 (4908-AD). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for ADAM22 (4908-AD) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for ADAM22 (4908-AD)

Discover related pathways, diseases and genes to ADAM22 (4908-AD). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ADAM22 (4908-AD)

Discover more about diseases related to ADAM22 (4908-AD).
 

Pathways for ADAM22 (4908-AD)

View related products by pathway.

PTMs for ADAM22 (4908-AD)

Learn more about PTMs related to ADAM22 (4908-AD).

Blogs on ADAM22

There are no specific blogs for ADAM22, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human ADAM22 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol ADAM22
Entrez
Uniprot