Recombinant Human Activin B Protein, CF

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED₅₀ for this effect is 0.200‑4.00 ng/mL.

2 μg/lane of Recombinant Human Activin B Protein (Catalog # 11517-AB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 10-13 kDa and 20-26 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Activin B Protein, CF Summary

• Details of Functionality: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED₅₀ for this effect is 0.200-4.00 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Activin B protein
  Gly293-Ala407
• Accession #: P09529.2
• N-terminal Sequence: Gly293
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 10-13 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Activin B Protein, CF

• activin AB beta polypeptide
• Activin B
• Activin beta-B chain
• INHBB
• inhibin beta B chain
• inhibin, beta B (activin AB beta polypeptide)
• inhibin, beta B
• Inhibin, beta-2

Background

Activins and inhibins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated or inhibited, respectively, pituitary follicle stimulating hormone (FSH) release. These proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Activins/inhibins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha  subunit and one of the various beta  subunits. Five beta subunits (mammalian beta _A, beta _B, beta _C, beta _E and  Xenopus beta _D) have been cloned. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A ( beta _A - beta _A), activin B ( beta _B­ - beta _B), activin AB ( beta _A ‑  beta _B), inhibin A ( alpha - beta _A) and inhibin B ( alpha - beta _B). At present, little is known about the contribution of the other beta  subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human beta B subunit is greater than 98% identical to mouse beta B, while the human and mouse alpha  subunits share approximately 80% identity. Similarly to other TGF-beta  family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated as type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling. Besides activins, Act RII has been shown to bind certain other TGF-beta superfamily members. Inhibin A has been shown to bind with low‑affinity to Act RII. The existence of a distinct inhibin-specific receptor and/or signal transduction pathway has been hypothesized.

1. Woodruff, T.K. (1998) Biochemical Pharmacology 55:953.
2. Ying, S.Y. et al. (1997) Proc. Soc. Exp. Biol. Med. 214:114.

Publications for Activin B/Inhibin beta B (11517-AB)(1)

Publication using 11517-AB

• Masihi, MB;Chambers, KR;Friedman, RA;Pampou, S;Gudenas, BL;Torrejon, J;Lee, C;Furnari, G;Chau, LQ;Gc, S;Lin, Y;Chapman, OS;Skowron, P;Garzia, L;Taylor, MD;Chavez, L;Ayrault, O;Northcott, PA;Karan, C;Wechsler-Reya, RJ; Ras-Responsive Element Binding Protein 1 regulates survival of Group 3 medulloblastoma bioRxiv : the preprint server for biology 2025-07-21 [PMID: 40777316] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • P09529.2()