Recombinant Cynomolgus/Rhesus Tyro3/Dtk His-tag Protein, CF

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey/Rhesus Macaque Tyro3/Dtk His-tag Protein (Catalog # 11301-DT) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Gas6 Protein (885-GSB) binds with an ED50 of 0.0600-0.600 μg/mL.

2 μg/lane of Recombinant Cynomolgus/Rhesus Tyro3/Dtk His-tag Protein (Catalog # 11301-DT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-64 kDa.

• Reactivity: Pm-Cm, RM
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus/Rhesus Tyro3/Dtk His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey/Rhesus Macaque Tyro3/Dtk His-tag (Catalog # 11301-DT) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Gas6 (Catalog # 885-GSB) binds with an ED₅₀ of 0.0600-0.600 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived Tyro3/Dtk protein
  Ala41-Ser428, with a C-terminal 6-His tag
• Accession #: XP_005559306.1
• N-terminal Sequence: Ala41
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-64 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus/Rhesus Tyro3/Dtk His-tag Protein, CF

• Brt
• BYK
• Dtk
• EC 2.7.10
• EC 2.7.10.1
• FLJ16467
• Rse
• Sky
• Tif
• TYRO3 protein tyrosine kinase
• Tyro3
• Tyrosine-protein kinase byk
• Tyrosine-protein kinase DTK
• tyrosine-protein kinase receptor TYRO3
• Tyrosine-protein kinase RSE
• Tyrosine-protein kinase SKY

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily (1, 2). The overall genomic structure of Dtk is virtually identical to that determined for the human UFO gene. This particular genomic organization is likely to have been duplicated and closely maintained throughout evolution (3). The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases (1). All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anti-coagulation factor protein S (1, 2). The binding affinities for Gas6 is in the order of Axl > Dtk > Mer (1). Gas6 binding induces tyrosine phosphorylation and down-stream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (1, 2). Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain (4). Within the ECD, cynomolgus monkey/rhesus macaque DTK shares 99% sequence identity with human DTK.

1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
3. Paula, M. et al. (1996) Genomics 31:13.
4. Philip, S. et al. (1994) Growth Factor 11:125.

Bioinformatics

• Uniprot:
  • XP_005559306.1()