Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF

When Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Chimera (10485-N2) is immobilized at 1 μg/mL (100 μL/well), Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera (9276-DN) binds with an ED50 of 0.1-0.6 μg/mL.

2 μg/lane of Recombinant Cynomolgus MonkeyNectin-2/CD112 Fc Chimera Protein (Catalog # 10485-N2) was resolved withSDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized byCoomassie® Blue staining, showing bands at kDa.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Nectin‑2/CD112 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera (Catalog # 9317-N2) binds with an ED₅₀ of 0.1-0.6 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Nectin-2/CD112 protein
  

  Cynomolgus Monkey (Gln32-Gly360) Accession # XP_005589607.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: XP_005589607.1
• N-terminal Sequence: No results obtained: Gln32 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 68-80 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey Nectin-2/CD112 Fc Protein, CF

• CD112 antigen
• CD112
• Herpes virus entry mediator B
• Herpesvirus entry mediator B
• herpesvirus entry protein B
• HVEB
• HVEBpoliovirus receptor-like 2
• MPH
• nectin 2
• Nectin2
• Nectin-2
• poliovirus receptor-related 2 (herpesvirus entry mediator B)
• poliovirus receptor-related protein 2
• PRR2
• PRR2nectin-2
• PVRL2
• PVRR2poliovirus receptor related 2

Background

Nectins are a small family of Ca⁺⁺-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. Based on its similarity with human Nectin-2, cynomolgus Nectin-2/CD112 (Nectin-2δ) is predicted to consist of a 329 amino acid (aa) extracellular region (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 157 aa cytoplasmic domain (2). Within the ECD, cynomolgus Nectin-2 shares 96% aa sequence identity with human Nectin-2. Alternative splicing of human Nectin-2 generates a short 60 kDa isoform with a 94 aa cytoplasmic tail (2). Nectin-2 localizes to adherens junctions between neurons, endothelial cells, epithelial cells, and fibroblasts (1, 3). It forms homodimers in cis, followed by dimers in trans (between cells) (3). It does not cis-dimerize with other Nectins but forms cis-dimers between its two splice forms. Notably, a Nectin-2 cis-dimer on one cell can heterodimerize with a Nectin-3 cis-dimer on a neighboring cell (3). Nectin-2 additionally binds to DNAM-1/CD226 on NK cells and triggers NK cell cytolytic activity (4, 5). Nectin-2 is known to bind pseudorabies virus and herpes simplex virus-2 (HSV-2), but not HSV-1 or poliovirus (3, 6). Nectin-2 is a component of cardiac intercalated discs and limits fibrosis and dysfunction resulting from pressure overload (7).

1. Samanta, D. and S.C. Almo (2015) Cell. Mol. Life Sci. 72:645.
2. Eberle, F. et al. (1995) Gene 159:267.
3. Struyf, F. et al. (2002) J. Virol. 76:12940.
4. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
5. Pende, D. et al. (2005) Mol. Immunol. 42:463.
6. Warner, M.S. et al. (1998) Virology 246:179.
7. Satomi-Kobayashi, S. et al. (2009) Hypertension 54:825.

Bioinformatics

• Uniprot:
  • XP_005589607.1()