Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein, CF

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Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
2 μg/lane of Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein (Catalog # 10530-L6) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, ...read more

Product Details

Summary
Reactivity Pm-CmSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 1-10 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Ly6E protein
Cynomolgus Monkey Ly6E
(Leu21-Ser101)
Accession # NP_001270930.1
3x GSL  Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu21
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW

35 kDa

.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38-50 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey Ly6E Fc Chimera Protein, CF

  • Ly6E
  • Ly-6E
  • lymphocyte antigen 6 complex, locus E
  • Retinoic acid-induced gene E protein
  • RIGE
  • RIGE9804
  • RIG-Ely-6E
  • SCA2
  • SCA2Ly-6E
  • SCA-2lymphocyte antigen 6E
  • Stem cell antigen 2
  • Thymic shared antigen 1
  • TSA1
  • TSA-1retinoic acid induced gene E

Background

Lymphocyte antigen 6E (Ly6E), also known as Stem cell antigen 2 (SCA-2) or Thymic shared antigen-1 (TSA-1), is a member of the lymphostromal cell membrane Ly6 protein superrfamily (1, 2). There are at least twenty different human Ly6 proteins that have been identified, and they are either secreted or GPI-anchor membrane-bound proteins (3). Cynomolgus Ly6E is synthesized as a precursor molecule that includes a signal peptide, a Ly6E chain, and a propeptide. Within the mature, main chain region, cynomolgus Ly6E shares 94% amino acid sequence identity with human Ly6E. Ly6E is involved in cell signalling transduction, cell adhesion, immune regulation and drug resistance, and is over-expressed in human malignancies, including head and neck squamous cell carcinomas and lung, and oesophageal cancers (4-7). Moreover, Ly6E over-expresses in gastric cancer cells, and is important for cell survival, proliferation and migration, and could be a novel oncogenic protein for efficient diagnosis and treatment of gastric cancer (2). LY6E has recently been shown to promote viral entry of some flaviviruses, including West Nile virus, Dengue virus, and Zika virus (8). Our in-house data indicate that Ly6E inhibits the human T cell activation, including IL-2, IFN-g secretions.
  1. Gumley, T.P. et al. (1995) Immunol. Cell Biol. 73:277.
  2. Lv, Y. et al. (2018) Cell. Physiol. Biochem. 45:1219.
  3. Kong, H.K. and J.H. Park. (2012) BMB Rep. 45:595.
  4. Luo, L. et al. (2016) Oncotarget 7:11165.
  5. Yeom, C.J. et al. (2016) Oncotarget 7:65837.
  6. AlHossiny, M. et al. (2016) Cancer Res. 76:3376.
  7. Asundi, J. et al. (2015) Clin Cancer Res. 21:3252.
  8. Mar, K.B. et al. (2018) Nat Commun. 9:3603.

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