Recombinant Cynomolgus Monkey B7-H3 Protein, CF

Recombinant Cynomolgus Monkey B7‑H3 inhibits IFN-gamma secretion by human T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1-10 μg/mL.

• Reactivity: Pm-Cm
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cynomolgus Monkey B7-H3 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 1-10 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived cynomolgus monkey B7-H3 protein
  Gly27 & Leu29-Thr461, with a C-terminal 6-His tag
• Accession #: XP_015308534
• N-terminal Sequence: Gly27 & Leu29
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 48 kDa (Gly27) & 47 kDa (Leu29).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65 - 80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cynomolgus Monkey B7-H3 Protein, CF

• B7H3
• B7-H3
• B7H34Ig-B7-H3
• B7-H3B7 homolog 3
• CD276 antigen
• CD276 molecule
• CD276
• Costimulatory molecule

Background

Cynomolgus Monkey B7 homolog 3 (B7-H3), also known as CD276 antigen, is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses (1-3). Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C-like domains with a short cytoplasmic tail. Among the family members there is about
20‑40% amino acid (aa) sequence identity. Cynomolgus monkey B7-H3 shares approximately 97% and 88% aa sequence identity with human and mouse B7-H3 respectively. B7-H3 mRNA is found in various normal tissues and in several tumor cell lines, but is not detectable in peripheral blood mononuclear cells (PBMCs). Inflammatory cytokines, such as IFN gamma , and a combination of phorbol myristate acetate (PMA) and ionomycin induce expression of B7-H3 protein on dendritic cells (DCs) and monocytes (4). The receptor(s) for B7-H3 remains unknown. Both murine and human B7-H3 fusion proteins fail to bind to resting T cells, but can recognize activated T cells which stimulated with PHA or ConA, indicating that B7-H3 receptors are induced upon T cell activation (5). B7-H3 was reported to act as a
co-stimulatory regulator to enhance the proliferation of both CD4⁺ and CD8⁺ T cells, the induction of cytotoxic T cells, and IFN-gamma production in the presence of TCR signaling (1). B7‑H3 was also reported to play an inhibitory role on T-cell activation. The inhibition may govern through nuclear factor of activated T cells (NFAT),
NF‑ kappa B, and AP-1 factors, three major signaling pathways through which TCR regulates gene transcription, which suggesting that B7‑H3 might have more than one receptor on T cells (6). B7-H3 protein expresses on a wide variety of cancers, including stomach, lung, prostate, kidney, ovary, pancreas, liver, bladder and breast. The role of B7-H3 in anti-tumor immunity is controversial, because B7-H3 has conflicting costimulatory and coinhibitory functions (7).

1. Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
3. Coyle, A. and J.Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
4. Kyung H. Yi and Lieping Chen. (2009) Immunol Rev. 229:145.
5. Sun M, et al. (2002) J Immunol. 168:6294.
6. Hofmeyer KA, et al. (2008) Proc Natl Acad Sci USA. 105:10277.
7. Ni L and Dong C. (2017) Immunol Rev. 276:52.

Bioinformatics

• Uniprot:
  • XP_015308534()