Recombinant Cotton Rat IL-13 Protein, CF

• Reactivity: Cr
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Cotton Rat IL-13 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 3 ‑ 18 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived cotton rat IL-13 protein
  Met1-His133, with a C-terminal 5-His tag
• Accession #: AAP93198
• N-terminal Sequence: Gly21
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15-30 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Cotton Rat IL-13 Protein, CF

• ALRHMGC116789
• BHR1interleukin-13
• IL13
• IL-13
• IL-13MGC116788
• interleukin 13
• MGC116786
• NC30
• P600

Background

IL‑13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4⁺ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1 ‑ 4). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha ‑helix configuration (5, 6). Mature cotton rat IL‑13 shares 59%, 74%, and 72% amino acid sequence identity with human, mouse, and rat IL‑13, respectively. Despite the low homology, it exhibits cross‑species activity between human, mouse, and rat (7, 8). IL‑13 has diverse activities on numerous cell types (9). On macrophages, IL‑13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL‑13 induces immunoglobulin class switching to IgE, up‑regulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL‑13 up‑regulates IL‑6 while down‑regulating IL‑1 and TNF‑ alpha production by fibroblasts and endothelial cells. IL‑13 binds with low affinity to IL‑13 R alpha 1, triggering IL‑13 R alpha 1 association with IL‑4 R alpha . This high affinity receptor complex also functions as the type 2 IL‑4 receptor complex (10, 11). Additionally, IL‑13 binds with high affinity to IL‑13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (12 ‑ 15). IL‑13 R alpha 2 regulates the bioavailability of both IL‑13 and IL‑4 and is overexpressed in glioma and several bronchial pathologies (11, 16, 17). Compared to wild type IL‑13, the atopy‑associated R110Q variant of IL‑13 elicits increased responsiveness from eosinophils that express low levels of IL‑13 R alpha 2 (18).

1. Wills-Karp, M. (2004) Immunol. Rev. 202:175.
2. Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
3. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. 90:3735.
4. Blanco, J.C.G. et al. (2004) J. Interferon Cytokine Res. 24:21.
5. Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
6. Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
7. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
8. Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
9. Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
10. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
11. Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
12. Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
13. Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
14. Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
15. Tabata, Y. et al. (2007) J. Immunol. 177:7905.
16. Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
17. Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
18. Andrews, A.-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.

Bioinformatics

• Uniprot:
  • AAP93198()