RCN1 Antibody

Images

 
Western Blot: RCN1 Antibody [NB500-256] - Whole cell lysate (5 and 15 ug) from HeLa cells, stained with RCN1 Antibody diluted at 0.2 ug/ml.
Immunohistochemistry: RCN1 Antibody [NB500-256] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RCN1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: RCN1 Antibody [NB500-256] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-Reticulocalbin used at a dilution of 1:250. Detection: DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

RCN1 Antibody Summary

Immunogen
A synthetic peptide that maps to a region between residues 1 and 75 of human Reticulocalbin-1, EF-hand calcium binding domain using the numbering given in Swiss-Prot entry Q15293 (GeneID 5954).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RCN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:250
  • Immunoprecipitation 3-5 ug/mg lysate
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
RCN1 Lysate (NBL1-15246)
Publications
Read Publication using NB500-256.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RCN1 Antibody

  • FLJ37041
  • FLJ55835
  • PIG20
  • proliferation-inducing gene 20
  • RCAL
  • RCN
  • reticulocalbin 1, EF-hand calcium binding domain
  • reticulocalbin-1

Background

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RCN1 Antibody (NB500-256)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RCN1
Entrez
Uniprot