Calumenin Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Calumenin Antibody [NBP1-90348] - Immunofluorescent staining of human cell line U-251 MG shows localization to endoplasmic reticulum.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Staining of human endometrium shows strong cytoplasmic - membranous positivity in glandular cells.

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.

Analysis in human cell line SK-MEL-30.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Calumenin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Calumenin Antibody - BSA Free (NBP1-90348) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Calumenin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TKEEFTAFLHPEEYDYMKDIVVQETMEDIDKNADGFIDLEEYIGDMYSHDGNTDEPEWVKTEREQFVEFRDKNRDGKMDKEETKDWILPSDYDHAEAEARHLVYESDQNKDGKLTKEEIVDKYD
• Predicted Species: Mouse (99%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CALU
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Calumenin Protein (NBP1-90348PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Calumenin Antibody - BSA Free

• calumenin
• crocalbin
• crocalbin-like protein
• FLJ90608
• IEF SSP 9302
• multiple EF-hand protein

Background

The product of this gene is a calcium-binding protein localized in the endoplasmic reticulum (ER) and it is involved in such ER functions as protein folding and sorting. This protein belongs to a family of multiple EF-hand proteins (CERC) that include reticulocalbin, ERC-55, and Cab45 and the product of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Calumenin Antibody (NBP1-90348)(1)

Publication using NBP1-90348

• Vasiljevic M, Heisler FF, Hausrat TJ et al. Spatio-temporal expression analysis of the calcium-binding protein calumenin in the rodent brain. Neuroscience 2012-01-01 [PMID: 22178608]

Bioinformatics

• Gene Symbol: CALU