RBM8A Antibody

Western Blot: RBM8A Antibody [NBP3-13019] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RBM8A antibody (NBP3-13019) diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: RBM8A Antibody [NBP3-13019] - RBM8A antibody detects RBM8A protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RBM8A protein stained by RBM8A antibody (NBP3-13019) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Immunohistochemistry-Paraffin: RBM8A Antibody [NBP3-13019] - RBM8A antibody detects RBM8A protein at cytoplasm and nucleus in rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. RBM8A antibody (NBP3-13019) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: RBM8A Antibody [NBP3-13019] - RBM8A antibody detects RBM8A protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. RBM8A antibody (NBP3-13019) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Rt, Mu, Bv, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RBM8A Antibody Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RBM8A. The exact sequence is proprietary.
• Predicted Species: Mouse (100%), Zebrafish (93%), Bovine (98%), Xenopus (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM8A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Japanese Medaka (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RBM8A Antibody

• Binder of OVCA1-1
• BOV-1
• BOV-1A
• BOV-1B
• BOV-1C
• MDS014
• RBM8BRBM8
• ribonucleoprotein RBM8
• Ribonucleoprotein RBM8A
• RNA binding motif protein 8A
• RNA binding motif protein 8B
• RNA-binding motif protein 8A
• RNA-binding protein 8A
• RNA-binding protein Y14
• Y14
• ZNRP
• ZRNP1

Background

Pre-mRNA splicing appears to play an important role in regulation of gene expression. It has been shown in a number of studies that during the splicing process, specific proteins are recruited to the mRNA and assemble into a complex of mRNA-protein near the exon-exon junction. This complex is termed mRNA-protein complex (mRNP), or the exon-exon junction complex. Several proteins were found to be present in this complex including Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK. Y14 is a 174 amino acid RNA-binding protein that contains one RNA-binding domain in the middle of the protein. Y14 binds to the spliced mRNAs in the nucleus and is exported with the mRNA to the cytoplasm. Similar to other proteins in the mRNA-protein complex, Y14 can interact with the mRNA export factor TAP and enhance the export of the mRNAs. Y14 binds to different mRNAs approx.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM8A