RBM8A Antibody

Western Blot: RBM8A Antibody [NB100-55326] - Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibody used at for WB at 0.04 ug/ml.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RBM8A Antibody Summary

• Description: Novus Biologicals Rabbit RBM8A Antibody (NB100-55326) is a polyclonal antibody validated for use in WB and IP. Anti-RBM8A Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human RNA binding motif protein 8A using the numbering given in entry NP_005096.1 (GeneID 9939).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM8A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot 1:2000-1:10000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  RBM8A Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBM8A Antibody

• Binder of OVCA1-1
• BOV-1
• BOV-1A
• BOV-1B
• BOV-1C
• MDS014
• RBM8BRBM8
• ribonucleoprotein RBM8
• Ribonucleoprotein RBM8A
• RNA binding motif protein 8A
• RNA binding motif protein 8B
• RNA-binding motif protein 8A
• RNA-binding protein 8A
• RNA-binding protein Y14
• Y14
• ZNRP
• ZRNP1

Background

This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RBM8A Antibody (NB100-55326)(3)

Publications using NB100-55326

• Sanchez G, Bondy-Chorney E, Laframboise J et al. A novel role for CARM1 in promoting nonsense-mediated mRNA decay: potential implications for spinal muscular atrophy. Nucleic Acids Res. 2015-12-09 [PMID: 26656492] (WB, Mouse)
• Chazal PE, Daguenet E, Wendling C et al. EJC core component MLN51 interacts with eIF3 and activates translation Proc Natl Acad Sci U S A 2013-03-25 [PMID: 23530232] (WB, Human)
• Barbosa I, Haque N, Fiorini F et al. Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly Nat Struct Mol Biol 2012-09-09 [PMID: 22961380] (WB, Human)

Bioinformatics

• Gene Symbol: RBM8A
• Entrez:
  • 9939(Human)
  • 60365(Mouse)
• Uniprot:
  • Q9Y5S9(Human)