RBM3 Antibody (CL0296) - Azide and BSA Free

Analysis in human tonsil and liver tissues using NBP3-43899 antibody. Corresponding RBM3 RNA-seq data are presented for the same tissues.

Staining of human breast cancer shows moderate nuclear positivity in tumor cells.

Lane 1: Marker [kDa]Lane 2: Human cell line RT-4

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human colorectal cancer shows weak to moderate nuclear positivity in a subset of tumor cells.

Staining of human rectum shows moderate nuclear positivity in glandular cells.

Staining of human tonsil shows moderate nuclear positivity in lymphoid cells, mainly in germinal centra.

Staining of U-2 OS cells using the anti-RBM3 monoclonal antibody) .

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RBM3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0296
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RBM3 Antibody (CL0296) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RBM3 Antibody (CL0296) - Azide and BSA Free (NBP2-52906) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P98179, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBM3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for RBM3 Antibody (CL0296) - Azide and BSA Free

• IS1-RNPL
• putative RNA-binding protein 3
• RNA binding motif (RNP1, RRM) protein 3
• RNA binding motif protein 3
• RNA-binding motif protein 3
• RNPL

Background

RBM3 is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM3