RBM15 Antibody (6B1) - BSA Free

Immunocytochemistry/ Immunofluorescence: RBM15 Antibody (6B1) [NBP3-43026] - Immunofluorescent analysis of Hela cells, using (RBM15 antibody) at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

Immunoprecipitation: RBM15 Antibody (6B1) [NBP3-43026] - IP result of anti-RBM15(IP:FNab07163, 4ug; Detection:FNab07163 1:500) with HEK-293 cells lysate 2600 ug

Immunohistochemistry: RBM15 Antibody (6B1) [NBP3-43026] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (RBM15 antibody) at dilution of 1:500

Western Blot: RBM15 Antibody (6B1) [NBP3-43026] - HeLa cells were subjected to SDS PAGE followed by western blot with (RBM15 antibody) at dilution of 1:1000

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: 6B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

RBM15 Antibody (6B1) - BSA Free Summary

• Immunogen: RNA binding motif protein 15 - (Uniprot# Q96T37)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBM15
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry 1:200 - 1:800
  • Immunoprecipitation 1:500 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 107 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for RBM15 Antibody (6B1) - BSA Free

• FLJ12479
• one twenty two protein
• one twenty-two
• One-twenty two protein 1
• putative RNA-binding protein 15
• RNA binding motif protein 15
• RNA-binding motif protein 15
• SPEN

Background

Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signalingpathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 (PubMed16129689)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM15