RBM10 Antibody

Western Blot: RBM10 Antibody [NB100-55265] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-55265 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). RBM10 was also immunoprecipitated by another rabbit anti-RBM10 antibody, which recognizes an upstream epitope.

Immunohistochemistry-Paraffin: RBM10 Antibody [NB100-55265] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- RBM10 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RBM10 Antibody Summary

• Description: Novus Biologicals Rabbit RBM10 Antibody (NB100-55265) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 880 and the C-terminus (residue 930) of human RNA binding motif protein 10 using the numbering given in entry NP_005667.2 (GeneID 8241).
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBM10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ul/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  RBM10 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RBM10 Antibody

• DXS8237ERNA-binding motif protein 10
• G patch domain-containing protein 9
• GPATC9TARPS
• GPATCH9MGC1132
• KIAA0122RNA-binding protein S1-1
• MGC997
• RNA binding motif protein 10
• RNA-binding protein 10
• S1-1
• ZRANB5

Background

RBM10 contains an RNA recognition motif found in a variety of RNA binding proteins, including various hnRNP proteins, proteins implicated in regulation of alternative splicing, and protein components of snRNPs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBM10
• Entrez:
  • 8241(Human)
• Uniprot:
  • NP_005667.2(Human)