RBBP9 Antibody - BSA Free

Western Blot: RBBP9 Antibody [NBP3-38095] - Western blot analysis of various lysates using RBBP9 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Immunohistochemistry: RBBP9 Antibody [NBP3-38095] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using RBBP9 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: RBBP9 Antibody [NBP3-38095] - Immunohistochemistry analysis of paraffin-embedded Rat kidney using RBBP9 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: RBBP9 Antibody [NBP3-38095] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using RBBP9 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: RBBP9 Antibody [NBP3-38095] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RBBP9 Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RBBP9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RBBP9 Antibody - BSA Free (NBP3-38095) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human RBBP9 (NP_006597.2).
  
  Sequence:
  MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBBP9
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for RBBP9 Antibody - BSA Free

• B5T-overexpressed gene protein
• Bog
• BOGB5T overexpressed gene protein
• EC 3.-
• Protein BOG
• putative hydrolase RBBP9
• RBBP10
• RBBP-10
• RBBP-9
• retinoblastoma binding protein 9
• Retinoblastoma-binding protein 10
• Retinoblastoma-binding protein 9MGC9236
• retinoma-binding protein 9

Background

RBBP9 is encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBBP9