RbAp46 Antibody

Western Blot: RbAp46 Antibody [NB120-3535] - Analysis of RbAp46 on 1) Du-145 2) LNCaP and 3) Hep-G2 cell extracts.

Immunocytochemistry/Immunofluorescence: RbAp46 Antibody [NB120-3535] - Immunolocalization of RbAp46 in HeLa cells.

Western Blot: RbAp46 Antibody [NB120-3535] - Figure 1: IF of RbAp46 in HeLa cells. Figure 2: WB of RbAp46 on 1) Du-145 2) LNCaP and 3) Hep-G2 cell extracts.

• Reactivity: Hu, Mu, Fi, Rt, Xp
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RbAp46 Antibody Summary

• Description: Novus Biologicals Rabbit RbAp46 Antibody (NB120-3535) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-RbAp46 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to residues M(1) A S K E M F E D T V E E(13) C of human RbAp46.
• Localization: Nuclear.
• Specificity: NB120-3535 detects retinoblastoma protein (Rb) associated protein 46 (RbAp46) from human cells.
• Predicted Species: Rat (100%), Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RBBP7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1.0 ug/ml
  • Simple Western 1:50
  • Western Blot 0.5 ug/ml
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in Blood, separated by Size, antibody dilution of 1:50
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (84%). Mouse reactivity reported in scientific literature (PMID: 14645126).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RbAp46 Antibody

• G1/S transition control protein-binding protein RbAp46
• Histone acetyltransferase type B subunit 2
• histone-binding protein RBBP7
• MGC138867
• Nucleosome-remodeling factor subunit RBAP46
• RbAp46
• RBBP-7
• retinoblastoma binding protein 7
• Retinoblastoma-binding protein 7MGC138868
• Retinoblastoma-binding protein p46
• retinoblastoma-binding protein RbAp46

Background

Retinoblastoma protein (Rb) associated protein 46 (RbAp 46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 46 shares about 75% sequence identity with the closely related RbAp 48, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp 46 has also been demonstrated to be up-regulated by the Wilms' tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RbAp46 Antibody (NB120-3535)(4)

Publications using NB120-3535

• Dubey, N, Hoffman, J F Et al. The ESC/E(Z) complex, an effector of response to ovarian steroids, manifests an intrinsic difference in cells from women with premenstrual dysphoric disorder. Mol Psychiatry 2017-08-01 [PMID: 28044059] (WB, Human)
• Hong Z, Jiang J, Lan L et al. A polycomb group protein, PHF1, is involved in the response to DNA double-strand breaks in human cell. Nucleic Acids Res. 2008-04-01 [PMID: 18385154]
• Zoltewicz JS, Stewart NJ, Leung R et al. Atrophin 2 recruits histone deacetylase and is required for the function of multiple signaling centers during mouse embryogenesis. Development. 2004-01-01 [PMID: 14645126]
• Guan LS, Rauchman M, Wang ZY et al. Induction of Rb-associated protein (RbAp46) by Wilms' tumor suppressor WT1 mediates growth inhibition. J Biol Chem. 1998-10-01 [PMID: 9765217]

Bioinformatics

• Gene Symbol: RBBP7