RB1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: RB1 Antibody [NBP2-20127] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rb stained by Rb antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Human colon carcinoma. Rb antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Immunoprecipitation: RB1 Antibody [NBP2-20127] - HeLa whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of retinoblastoma 1 protein by 4 ug Rb1 antibody 7.5 % SDS-PAGE The immunoprecipitated retinoblastoma 1 protein was detected by Rb1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Western Blot: RB1 Antibody [NBP2-20127] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RB1 antibody (NBP2-20127) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: RB1 Antibody [NBP2-20127] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with RB1 antibody (NBP2-20127) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Rt, Po, Bv, RM
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

RB1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RB1 Antibody - BSA Free (NBP2-20127) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-RB1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human RB1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (96%), Porcine (96%), Bovine (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot Assay dependent
• Theoretical MW: 106 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for RB1 Antibody - BSA Free

• OSRC
• osteosarcoma
• p105-Rb
• pp110
• pRb
• RB
• RB1
• retinoblastoma 1
• retinoblastoma suspectibility protein
• retinoblastoma-associated protein

Background

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for RB1 Antibody (NBP2-20127)(1)

Publication using NBP2-20127

• Weber M, Nguyen MB, Li MY et al. Merkel cell polyomavirus T-antigen-mediated reprogramming in adult Merkel cell progenitors The Journal of investigative dermatology 2023-05-29 [PMID: 37257637] (IHC, Mouse)

Bioinformatics

• Gene Symbol: RB1