Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit

• Reactivity: Rt
• Applications: ELISA
• Conjugate: HRP

Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary

• Background: The Quantikine Rat IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant rat IL-1 alpha accurately. Results obtained using... natural rat IL-1 alpha showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-1 alpha.
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• Specificity: Natural and recombinant rat IL-1 alpha
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il1a

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rat IL-1 alpha/IL-1F1 Quantikine ELISA Kit

• BAF
• Hematopoietin-1
• IL1 alpha
• IL-1 alpha
• IL1
• IL1A
• IL-1A
• IL1-ALPHA
• IL1F1
• IL-1F1
• IL1F1hematopoietin-1
• interleukin 1, alpha
• interleukin-1 alpha
• LAF
• LEM
• preinterleukin 1 alpha
• pro-interleukin-1-alpha

Background

IL-1 alpha (Interleukin-1 alpha) is a cytokine that is induced during microbial exposure and promotes the release of multiple inflammatory mediators and acute phase proteins. It also supports hematopoietic stem cell expansion, promotes neutrophil mobilization from the bone marrow, and promotes osteoclast formation. IL-1 alpha signals through a receptor complex composed of IL-1 RI and IL-1 RAcP. It also binds the decoy receptor IL-1 RII which associates with IL-1 RAcP but does not transduce signaling.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-1 alpha/IL-1F1 (RRA00)(6)

Publications using RRA00

• L Manzano-Pe, V Guarner-La, ME Soto, E Díaz-Díaz, S Caballero-, R Díaz-Torre, FL Rodríguez-, I Pérez-Torr Excessive Consumption Hibiscus sabdariffa L. Increases Inflammation and Blood Pressure in Male Wistar Rats via High Antioxidant Capacity: The Preliminary Findings Cells, 2022-09-06;11(18):. 2022-09-06 [PMID: 36139349] (Rat)
• M Li, H Yu, H Pan, X Zhou, Q Ruan, D Kong, Z Chu, H Li, J Huang, X Huang, A Chau, W Xie, Y Ding, P Yao Nrf2 Suppression Delays Diabetic Wound Healing Through Sustained Oxidative Stress and Inflammation Front Pharmacol, 2019-09-20;10(0):1099. 2019-09-20 [PMID: 31616304] (Rat)
• B Nieto-Lima, A Cano-Martí, ME Rubio-Ruiz, I Pérez-Torr, V Guarner-La Age-, Gender-, andin VivoDifferent Doses of Isoproterenol Modifyin VitroAortic Vasoreactivity and Circulating VCAM-1 Front Physiol, 2018-01-24;9(0):20. 2018-01-24 [PMID: 29416512] (Mouse)
• B Argibay, J Trekker, U Himmelreic, A Beiras, A Topete, P Taboada, M Pérez-Mato, A Vieites-Pr, R Iglesias-R, J Rivas, AM Planas, T Sobrino, J Castillo, F Campos Intraarterial route increases the risk of cerebral lesions after mesenchymal cell administration in animal model of ischemia Sci Rep, 2017-01-16;7(0):40758. 2017-01-16 [PMID: 28091591] (Rat)
• Kondo M, Yamato M, Takagi R, Namiki H, Okano T Membrane-Permeable Calpain Inhibitors Promote Rat Oral Mucosal Epithelial Cell Proliferation by Inhibiting IL-1alpha Signaling. PLoS ONE, 2015-07-31;10(7):e0134240. 2015-07-31 [PMID: 26230502] (Rat)
• Winnall WR, Okuma Y, Saito K Regulation of interleukin 1alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats. Mol. Cell. Endocrinol., 2009-02-28;307(1):169-75. 2009-02-28 [PMID: 19524137] (Rat)

FAQs for IL-1 alpha/IL-1F1 (RRA00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Il1a