RASGRP3 Antibody

Western Blot: RASGRP3 Antibody [NBP1-31501] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.

Immunohistochemistry-Paraffin: RASGRP3 Antibody [NBP1-31501] - Paraffin-embedded CA922 xenograft, using antibody at 1:100 dilution.

Western Blot: RASGRP3 Antibody [NBP1-31501] - Analysis of NBP1-31501 on JurKat, with 7.5% SDS PAGE, diluted at 1:1000.

Product Discontinued

RASGRP3 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human RASGRP3. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RASGRP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%) .

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for RASGRP3 Antibody

• Calcium and DAG-regulated guanine nucleotide exchange factor III
• GRP3KIAA0846ras guanyl-releasing protein 3
• Guanine nucleotide exchange factor for Rap1
• RAS guanyl releasing protein 3 (calcium and DAG-regulated)

Background

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RASGRP3
• Entrez:
  • 25780(Human)
• Uniprot:
  • Q8IV61(Human)