RASGRP3 Antibody

Product Discontinued

RASGRP3 Antibody Summary

• Immunogen: Sythetic peptide GSSGLGKAATLDE-C, representing the N-Terminus of the human protein sequence according to NP_733772.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RASGRP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA 1:32000
• Application Notes: Preliminary experiments gave an approx 48kDa band in Human Brain and Kidney lysates.
• Control:

  RASGRP3 Overexpression Lysate

Reactivity Notes

Cross reacts with Human. Expected from sequence similarity: Mouse, Rat, Dog, Pig and Cow.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for RASGRP3 Antibody

• Calcium and DAG-regulated guanine nucleotide exchange factor III
• GRP3KIAA0846ras guanyl-releasing protein 3
• Guanine nucleotide exchange factor for Rap1
• RAS guanyl releasing protein 3 (calcium and DAG-regulated)

Background

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RASGRP3
• Entrez:
  • 25780(Human)
• Uniprot:
  • NP_733772.(Human)