RAPGEF3 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

RAPGEF3 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human RAPGEF3.
  
  

  Source: E. coli

  Amino Acid Sequence: TYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLREQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQ

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: RAPGEF3
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-33395.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for RAPGEF3 Recombinant Protein Antigen

• 9330170P05Rik
• bcm910
• cAMP-GEFI
• cAMP-regulated guanine nucleotide exchange factor I
• CGEF1
• EPAC 1
• EPAC
• EPAC1
• EPACRAP guanine-nucleotide-exchange factor (GEF) 3
• Exchange factor directly activated by cAMP 1
• Exchange protein directly activated by cAMP 1
• HSU79275
• MGC21410
• Rap guanine nucleotide exchange factor (GEF) 3
• rap guanine nucleotide exchange factor 3
• Rap1 guanine-nucleotide-exchange factor directly activated by cAMP
• RAPGEF3

Background

RAPGEF3, also known as Rap guanine nucleotide exchange factor 3, is a 104 kDa 923 amino acid protein with two shorter isoforms produced by alternative splicing. The gene is necessary for rearranging actin at cell to cell junctions. RAPGEF3 is currently being studied for its involvement with leukemia, melanoma, pancreatitis, thyroiditis, carcinoma, prostatitis and neuronitis. RAPGEF3 is linked to the chemotaxis, cell adhesion, insulin secretion, and immune system pathways where it interacts with GNAI2, GNAI1, PDE3B, RAP1A and RAP1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RAPGEF3