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RAPGEF3 Antibody (JM63-29)

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Western Blot: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of Epac1 on mouse kidney tissue lysate using anti-Epac1 antibody at 1/1,000 dilution.
Western Blot: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of Epac1 on mouse kidney tissue lysate using anti-Epac1 antibody at 1/1,000 dilution.
Immunohistochemistry-Paraffin: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of paraffin-embedded mouse kidney tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Epac1 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
JM63-29
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RAPGEF3 Antibody (JM63-29) Summary

Immunogen
Recombinant protein within human Epac1 aa 700-900
Localization
Endomembrane system.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RAPGEF3
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for RAPGEF3 Antibody (JM63-29)

  • 9330170P05Rik
  • bcm910
  • cAMP-GEFI
  • cAMP-regulated guanine nucleotide exchange factor I
  • CGEF1
  • EPAC 1
  • EPAC1
  • EPACRAP guanine-nucleotide-exchange factor (GEF) 3
  • Exchange factor directly activated by cAMP 1
  • Exchange protein directly activated by cAMP 1
  • HSU79275
  • MGC21410
  • Rap guanine nucleotide exchange factor (GEF) 3
  • rap guanine nucleotide exchange factor 3
  • Rap1 guanine-nucleotide-exchange factor directly activated by cAMP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: TERF2IP Antibody [NB100-292] - Detection of Human RAP1 by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAP1 antibody NB100-292 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: TERF2IP Antibody [NB100-292] - Sections of human lung carcinoma.~~ Antibody: Affinity purified rabbit anti-RAP1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: TERF2IP Antibody [NB100-292] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinitypurified rabbit anti-RAP1 antibody used for IP at 6 ug per reaction. RAP1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-RAP1 antibody. For blotting immunoprecipitated RAP1, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(19 Publications)
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Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.
Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).
Species: Hu, Mu
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Western Blot: Rabex5 Antibody [NBP1-49938] - Detection of Human and Mouse RABGEF1 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RABGEF1 was also immunoprecipitated by rabbit anti-RABGEF1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated RABGEF1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunohistochemistry: Rabex5 Antibody [NBP1-49938] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- RABGEF1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
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Western Blot: Epac2 Antibody [NBP2-32009] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: Epac2 Antibody [NBP2-32009] - Staining of human cell line RT4 shows localization to focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Epac2 Antibody [NBP2-32009] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
(1 Review)
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Immunocytochemistry/Immunofluorescence: RASGRF1 Antibody [NBP1-85727] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP1-85727] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
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Applications: ICC/IF, IHC, IHC-P
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Western Blot: LRPAP Antibody [NBP1-89334] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue
Immunocytochemistry/Immunofluorescence: LRPAP Antibody [NBP1-89334] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LRPAP Antibody [NBP1-89334] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
(2 Publications)
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Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Immunohistochemistry-Frozen: CREB [p Ser133] Antibody [NB300-273] - IHC Analysis of pCREB S133 (red) in frozen sections from mouse piriform cortex. Cell nuclei are visualized with DAPI DNA stain (blue). NB300-273 was used at a dilution of 1:1000 and incubated for 48 hours at 4C. No antigen retrieval step was performed.
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Immunohistochemistry-Frozen: GEF-H1 Antibody [NBP2-21577] - Frozen-sectioned mouse mouse cerebellum. Green: GEF-H1 stained by GEF-H1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
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Western Blot: SLC2A4RG Antibody (4C10) [H00056731-M02] - SLC2A4RG monoclonal antibody (M02), clone 4C10 Analysis of SLC2A4RG expression in HeLa.
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RAPGEF3 Antibody
(JM63-29)
NBP2-75641
RAPGEF3 Antibody (JM63-29)
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP

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Bioinformatics

Gene Symbol RAPGEF3