RAPGEF3 Antibody (JM63-29)

Western Blot: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Western blot analysis of RAPGEF3 on PC3 cell lysates using anti-RAPGEF3 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of paraffin-embedded mouse kidney tissue using anti-Epac1 antibody. Counter stained with hematoxylin.

Western Blot: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of Epac1 on mouse kidney tissue lysate using anti-Epac1 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of paraffin-embedded human prostate tissue using anti-Epac1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RAPGEF3 Antibody (JM63-29) [NBP2-75641] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Epac1 antibody. Counter stained with hematoxylin.

Product Discontinued

RAPGEF3 Antibody (JM63-29) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human RAPGEF3 aa 874-923 / 923. (SwissProt: O95398 Human; SwissProt: Q8VCC8 Mouse)
• Localization: Endomembrane system.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RAPGEF3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:50
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RAPGEF3 Antibody (JM63-29)

• 9330170P05Rik
• bcm910
• cAMP-GEFI
• cAMP-regulated guanine nucleotide exchange factor I
• CGEF1
• EPAC 1
• EPAC
• EPAC1
• EPACRAP guanine-nucleotide-exchange factor (GEF) 3
• Exchange factor directly activated by cAMP 1
• Exchange protein directly activated by cAMP 1
• HSU79275
• MGC21410
• Rap guanine nucleotide exchange factor (GEF) 3
• rap guanine nucleotide exchange factor 3
• Rap1 guanine-nucleotide-exchange factor directly activated by cAMP
• RAPGEF3

Background

RAPGEF3, also known as Rap guanine nucleotide exchange factor 3, is a 104 kDa 923 amino acid protein with two shorter isoforms produced by alternative splicing. The gene is necessary for rearranging actin at cell to cell junctions. RAPGEF3 is currently being studied for its involvement with leukemia, melanoma, pancreatitis, thyroiditis, carcinoma, prostatitis and neuronitis. RAPGEF3 is linked to the chemotaxis, cell adhesion, insulin secretion, and immune system pathways where it interacts with GNAI2, GNAI1, PDE3B, RAP1A and RAP1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAPGEF3