RAP74 Antibody

Western Blot: RAP74 Antibody [NBP3-17975] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:3000.

Western Blot: RAP74 Antibody [NBP3-17975] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:3000.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.

TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 ug whole cell lysate/extract of TFIIF protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of TFIIF by 2.5 ug of TFIIF antibody (NBP3-17975) 7.5% SDS-PAGE The immunoprecipitated TFIIF protein was detected by TFIIF antibody (NBP3-17975) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

• Reactivity: Hu, Mu
• Applications: WB, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RAP74 Antibody Summary

• Description: Novus Biologicals Rabbit RAP74 Antibody (NBP3-17975) is a polyclonal antibody validated for use in WB, IP and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RAP74. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2F1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000

Reactivity Notes

Chimpanzee (97%), Rat (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RAP74 Antibody

• BTF4
• General transcription factor IIF 74 kDa subunit
• general transcription factor IIF subunit 1
• general transcription factor IIF, polypeptide 1, 74kDa
• TF2F1
• TFIIF
• TFIIF-alpha
• Transcription initiation factor IIF subunit alpha
• transcription initiation factor RAP74

Background

Enables several functions, including RNA polymerase II general transcription initiation factor activity; phosphatase activator activity; and promoter-specific chromatin binding activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; response to virus; and transcription initiation from RNA polymerase II promoter. Located in cell junction and nucleoplasm. Part of transcription factor TFIID complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: GTF2F1