RAP1A Antibody (5F8G2) - BSA Free

Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: RAP1A Antibody (5F8G2) [NBP2-37495] - Flow cytometric analysis of HeLa cells using RAP1A mouse mAb (green) and negative control (red).

Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 21 kDa)

ELISA: RAP1A Antibody (5F8G2) [NBP2-37495] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 5F8G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RAP1A Antibody (5F8G2) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human RAP1A expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAP1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 43.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for RAP1A Antibody (5F8G2) - BSA Free

• C21KG
• G-22K
• GTP-binding protein smg p21A
• KREV-1
• KREV1Ras-related protein Krev-1
• RAP1
• Rap1A
• RAP1A, member of RAS oncogene family
• RAS-related protein RAP1A
• ras-related protein Rap-1A
• SMGP21

Background

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAP1A