RAP1A Antibodies
We offer RAP1A Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each RAP1A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our RAP1A Antibodies can be used in a variety of model species: Bovine, Canine, Chicken, Guinea Pig, Hamster, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep, Xenopus. Use the list below to choose the RAP1A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our RAP1A polyclonal antibodies and browse our RAP1A monoclonal antibody catalog.
Alternate Names for RAP1A Antibodies
anti-RAP1A antibody, anti-C21KG antibody, anti-G-22K antibody, anti-GTP-binding protein smg p21A antibody, anti-KREV-1 antibody, anti-KREV1Ras-related protein Krev-1 antibody, anti-RAP1 antibody, anti-RAP1A, member of RAS oncogene family antibody, anti-ras-related protein Rap-1A antibody, anti-RAS-related protein RAP1A antibody, anti-SMGP21 antibody
9 Results for "rap1a" in Primary Antibodies
![Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Western-Blot-NBP2-22527-img0006.jpg "Western Blot: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of 25 ug of various whole cell lysate per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunocytochemistry-Immunofluorescence-NBP2-22527-img0003.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human colon carcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0005.jpg)
![Immunohistochemistry-Paraffin: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis showing staining in the cytoplasm and membrane of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunohistochemistry-Paraffin-NBP2-22527-img0004.jpg)
![Immunoprecipitation: RAP1A Antibody (1D4-1A3) [NBP2-22527] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 whole cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample BUffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D4-1A3-Immunoprecipitation-NBP2-22527-img0002.jpg)
![Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Western-Blot-NBP2-22526-img0001.jpg "Western Blot: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of 25 ug of various whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) showing staining in the in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0006.jpg)
![Immunohistochemistry: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 showing staining in the membrane of human kidney tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunohistochemistry-NBP2-22526-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA/TBST for 15 minutes at room temperature. Cells were probed with a Rap1 monoclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunocytochemistry-Immunofluorescence-NBP2-22526-img0003.jpg)
![Immunoprecipitation: RAP1A Antibody (1D2-1C64) [NBP2-22526] - Analysis of Rap1 was performed on NIH3T3 cells. Antigen-antibody complexes were formed by incubating 750ug of NIH3T3 cell lysate with 2ug of a Rap1 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Eluted sample (right lane) and 25ug of NIH3T3 control lysate (left lane) were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a Rap1 monoclonal antibody at a dilution of 1:500 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/RAP1A-Antibody-1D2-1C64-Immunoprecipitation-NBP2-22526-img0002.jpg)
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-10122024121014.jpg "Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-101220241224118.jpg)
![Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of lysates from wild type(WT) and RAP1A knockdown (KD) 293T cells, using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411552841.jpg)
![Immunohistochemistry: RAP1A Antibody [NBP3-35089] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] RAP1A Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411592829.jpg)
![Western Blot: RAP1A Antibody [NBP3-35089] - Western blot analysis of various lysates using [KO Validated] RAP1A Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35089_rabbit-rap1a-pab-1012202411535893.jpg)
![Immunocytochemistry/Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) [NBP2-50269] - Staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.](http://images.novusbio.com/fullsize/RAP1A-Antibody-RAP1-4C8-1-Immunofluorescence-NBP2-50269-img0001.jpg "Immunocytochemistry/Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) [NBP2-50269] - Staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.")
![Proximity Ligation Assay: RAP1A Antibody (3E1) [H00005906-M01] - Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/RAP1A-Antibody-3E1-Proximity-Ligation-Assay-H00005906-M01-img0002.jpg "Proximity Ligation Assay: RAP1A Antibody (3E1) [H00005906-M01] - Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: RAP1A Antibody (3E1) [H00005906-M01] - Detection limit for recombinant GST tagged RAP1A is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RAP1A-Antibody-3E1-Sandwich-ELISA-H00005906-M01-img0003.jpg)
![Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Western-Blot-NBP2-37495-img0003.jpg "Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: RAP1A Antibody (5F8G2) [NBP2-37495] - Flow cytometric analysis of HeLa cells using RAP1A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Flow-Cytometry-NBP2-37495-img0004.jpg)
![Western Blot: RAP1A Antibody (5F8G2) [NBP2-37495] - Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 21 kDa)](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-Western-Blot-NBP2-37495-img0002.jpg)
![ELISA: RAP1A Antibody (5F8G2) [NBP2-37495] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8G2-ELISA-NBP2-37495-img0001.jpg)
![Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Western-Blot-NBP2-37496-img0003.jpg "Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: RAP1A Antibody (5F8) [NBP2-37496] - Flow cytometric analysis of A431 cells using RAP1A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Flow-Cytometry-NBP2-37496-img0004.jpg)
![Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 43.2 kDa)](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-Western-Blot-NBP2-37496-img0002.jpg)
![ELISA: RAP1A Antibody (5F8) [NBP2-37496] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RAP1A-Antibody-5F8-ELISA-NBP2-37496-img0001.jpg)
![Western Blot: RAP1A Antibody [NBP1-32126] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAP1A antibody (NBP1-32126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32126_rabbit-polyclonal-rap1a-antibody-9102024201577.jpg "Western Blot: RAP1A Antibody [NBP1-32126] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RAP1A antibody (NBP1-32126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: RAP1A Antibody [NBP1-32126] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAP1A antibody (NBP1-32126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32126_rabbit-polyclonal-rap1a-antibody-9102024205621.jpg)
![Western Blot: RAP1A Antibody [NBP3-09344] - Western blot analysis of RAP1A in Mouse Testis lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/RAP1A-Antibody-Western-Blot-NBP3-09344-img0001.jpg "Western Blot: RAP1A Antibody [NBP3-09344] - Western blot analysis of RAP1A in Mouse Testis lysates. Antibody dilution at 1.0ug/ml")