Recombinant Human RanBP1 His Protein

SDS-Page: Recombinant Human RanBP1 Protein [NBP1-99058] - 15% SDS-PAGE (3ug)

SDS-Page: Recombinant Human RanBP1 Protein [NBP1-99058] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human RanBP1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-201 of Human RanBP1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAAAKD THEDHDTSTE NTDESNHDPQ FEPIVSLPEQ EIKTLEEDEE ELFKMRAKLF RFASENDLPE WKERGTGDVK LLKHKEKGAI RLLMRRDKTL KICANHYITP MMELKPNAGS DRAWVWNTHA DFADECPKPE LLAIRFLNAE NAQKFKTKFE ECRKEIEERE KKAGSGKNDH AEKVAEKLEA LSVKEETKED AEEKQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: RANBP1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 25.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human RanBP1 His Protein

• HTF9A
• MGC88701
• RAN binding protein 1
• Ran-binding protein 1
• RanBP1
• ran-specific GTPase-activating protein

Background

RAN binding protein 1, also known as RANBP1, belongs to a family of proteins that bind Ran GTPase which is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). Also, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN. Recombinant human RANBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RANBP1