Ran Antibody

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Western Blot: Ran Antibody [NB100-1442] - Detection of Ran in A431 cell lysate (RIPA buffer, 35 ug total protein per lane) using this antibody at 1 ug/ml. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Ran Antibody Summary

Immunogen
Peptide with sequence AAQGEPQVQFKLV-C corresponding to N-Terminus according to NP_006316.1.
Localization
Nuclear
Predicted Species
Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RAN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 25 kDa band observed in A431, HeLa, HepG2 and NIH3T3 lysates (calculated MW of 24.4 kDa band according to NP_006316.1).
Control
Jurkat Whole Cell Lysate
A-431 Whole Cell Lysate
Publications
Read Publication using NB100-1442.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ran Antibody

  • Androgen receptor-associated protein 24
  • ARA24Gsp1
  • GTPase Ran
  • GTP-binding nuclear protein Ran
  • guanosine triphosphatase Ran
  • member RAS oncogene family
  • OK/SW-cl.81
  • RAN, member RAS oncogene family
  • RanGTPase
  • Ras-like protein TC4
  • Ras-related nuclear protein
  • TC4

Background

Ran (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The Ran protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of Ran requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in Ran disrupt DNA synthesis. Because of its many functions, it is likely that Ran interacts with several other proteins. Ran regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. Ran could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of Ran-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Ran is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). Ran coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ran Antibody (NB100-1442)(1)


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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Ran Antibody (NB100-1442)

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Research Areas for Ran Antibody (NB100-1442)

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Blogs on Ran.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

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Bioinformatics

Gene Symbol RAN
Entrez
Uniprot