Recombinant Human RAIDD/CRADD His Protein

SDS-Page: RAIDD Protein [NBC1-18519] - CRADD, 24.9kDa (219aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human RAIDD/CRADD His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-199 of Human RAIDD/CRADD
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG LRKTMLMLDI LPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG DRLTGIPSHI LNSSPSDRQI NQLAQRLGPE WEPMVLSLGL SQTDIYRCKA NHPHNVQSQV VEAFIRWRQR FGKQATFQSL HNGLRAVEVD PSLLLHMLE

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CRADD
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 24.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human RAIDD/CRADD His Protein

• CASP2 and RIPK1 domain containing adaptor with death domain
• Caspase and RIP adapter with death domain
• caspase and RIP adaptor with death domain
• CRADD
• death domain containing protein CRADD
• death domain-containing protein CRADD
• MGC9163
• RAIDD
• RAIDDdeath adaptor molecule RAIDD
• RIP-associated ICH1/CED3-homologous protein with death domain
• RIP-associated protein with a death domain

Background

CRADD is a 22 kDa, widely-expressed cytosolic adaptor protein. CRADD is a death domain (CARD/DD) containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. Recombinant CRADD protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CRADD