Radixin Antibody (SC06-14)

Western Blot: Radixin Antibody (SC06-14) [NBP2-67584] - Western blot analysis of Radixin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-R

Immunocytochemistry/Immunofluorescence: Radixin Antibody (SC06-14) [NBP2-67584] - Staining Radixin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Radixin Antibody (SC06-14) [NBP2-67584] - Analysis of paraffin-embedded mouse placenta tissue using anti-Radixin antibody. Counter stained with hematoxylin.

Flow Cytometry: Radixin Antibody (SC06-14) [NBP2-67584] - Analysis of Hela cells with Radixin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Radixin Antibody (SC06-14) [NBP2-67584] - Analysis of Radixin on different lysates using anti-Radixin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: HepG2

Immunohistochemistry-Paraffin: Radixin Antibody (SC06-14) [NBP2-67584] - Analysis of paraffin-embedded human kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Radixin Antibody (SC06-14) [NBP2-67584] - Analysis of paraffin-embedded human tonsil tissue using anti-Radixin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Radixin Antibody (SC06-14) [NBP2-67584] - Analysis of paraffin-embedded mouse kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.

Product Discontinued

Radixin Antibody (SC06-14) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Radixin aa 389-433 / 583. (SwissProt: P35241 Human; SwissProt: P26043 Mouse; Unigene:224619 Rat)
• Localization: Cell membrane, Cytoplasm, Cleavage furrow.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RDX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Radixin Antibody (SC06-14)

• deafness, autosomal recessive 24
• DFNB24
• radixin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RDX