RAD54L Antibody (Rad54 4E3/1) - BSA Free

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: Rad54 4E3/1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RAD54L Antibody (Rad54 4E3/1) - BSA Free Summary

• Immunogen: Full length human RAD54L protein
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD54L
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100 - 1:2000
• Application Notes: This antibody is Cytof ready. Positive control(s): HeLa cell line.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RAD54L Antibody (Rad54 4E3/1) - BSA Free

• EC 3.6.1
• hHR54EC 3.6.4.-
• HR54
• hRAD54HRAD54
• RAD54 (S.cerevisiae)-like
• RAD54 homolog
• RAD54ADNA repair and recombination protein RAD54-like
• RAD54-like (S. cerevisiae)

Background

The protein encoded by the RAD54 gene belongs to the DEAD like helicase superfamily and shares similarity with Saccharomyces cerevisiae RAD54. RAD54 has been shown to play a role in homologous recombination related repair of DNA double strand breaks. The binding of this protein to double strand DNA induces a DNA topological change, which is thought to facilitate homologous DNA paring and stimulate DNA recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RAD54L Antibody (NB110-13364)(2)

Publications using NB110-13364

• Tang L, Deng L, Bai HX et al. Reduced expression of DNA repair genes and chemosensitivity in 1p19q codeleted lower-grade gliomas. J Neurooncol. [PMID: 29923053] (WB, Human)
• Srivastava V, Modi P, Tripathi V et al. BLM helicase stimulates the ATPase and chromatin-remodeling activities of RAD54. J Cell Sci 2009-09-01 [PMID: 19671661]

Bioinformatics

• Gene Symbol: RAD54L
• Entrez:
  • 8438(Human)