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RAD54L Antibody (Rad54 4E3/1) [DyLight 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
Rad54 4E3/1
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-13364AF350
Alexa Fluor 405
NB110-13364AF405
Alexa Fluor 488
NB110-13364AF488
Alexa Fluor 532
NB110-13364AF532
Alexa Fluor 594
NB110-13364AF594
Alexa Fluor 647
NB110-13364AF647
Alexa Fluor 700
NB110-13364AF700
Alexa Fluor 750
NB110-13364AF750
Biotin
NB110-13364B
DyLight 350
NB110-13364UV
DyLight 488
NB110-13364G
DyLight 550
NB110-13364R
DyLight 594
NB110-13364DL594
DyLight 650
NB110-13364C
DyLight 680
NB110-13364FR
DyLight 755
NB110-13364IR
HRP
NB110-13364H
Janelia Fluor 549
NB110-13364JF549
Janelia Fluor 646
NB110-13364JF646
Unconjugated
NB110-13364
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RAD54L Antibody (Rad54 4E3/1) [DyLight 405] Summary

Immunogen
Full length human Rad54 protein
Localization
Nuclear
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RAD54L
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for RAD54L Antibody (Rad54 4E3/1) [DyLight 405]

  • EC 3.6.1
  • hHR54EC 3.6.4.-
  • HR54
  • hRAD54HRAD54
  • RAD54 (S.cerevisiae)-like
  • RAD54 homolog
  • RAD54ADNA repair and recombination protein RAD54-like
  • RAD54-like (S. cerevisiae)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: ATRX Antibody [NBP1-83077] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATRX antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
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Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - Staining of RAD51 nuclear foci in U2OS cells using RAD51 14B4 antibody. Cells were pre-extracted with CSK buffer before fixation with 4% PFA. RAD51 14B4 was used at 1:1000 dultion. DAPI was used to counterstain the nucleus. Scale bar, 10 mciro-meter.
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Western Blot: RAD54B Antibody [NBP1-58233] - Human Brain lysate, concentration 0.2-1 ug/ml.
Immunocytochemistry/Immunofluorescence: RAD54B Antibody [NBP1-58233] - Antibody Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver Observed Staining: Nuclear in hepatocytes, very strong signal, wide tissue distribution
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Blooms Syndrome
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Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody (NB100-214, lot 4) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot 3). For blotting immunoprecipitated BLM, NB100-214 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.
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NBP1-90015
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(1D12/4)
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(1 Publication)
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Applications: WB, IP
ATM Antibody (2C1)
NB100-309
Western Blot: ATM Antibody (2C1) [NB100-309] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody (2C1) [NB100-309] diluted at 1:1000. Theoretical molecular weight 351 kDa.
Immunohistochemistry-Paraffin: ATM Antibody (2C1) [NB100-309] - Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody (2C1) [NB100-309] at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western Blot: ATM Antibody (2C1) [NB100-309] - Detection of human ATM protein using ATM antibody (2C1) [NB100-309] by western blot or immunoprecipitation. Theoretical molecular weight 351 kDa.
(3 Reviews)
(120 Publications)
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Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, KD
RAD54L Antibody
(Rad54 4E3/1) [DyLigh ...
NB110-13364V
RAD54L Antibody (Rad54 4E3/1) [DyLight 405]
Species: Hu
Applications: WB, Flow, ICC/IF, IHC

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FAQs for RAD54L Antibody (NB110-13364V) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-13364AF350
Alexa Fluor 405 NB110-13364AF405
Alexa Fluor 488 NB110-13364AF488
Alexa Fluor 532 NB110-13364AF532
Alexa Fluor 594 NB110-13364AF594
Alexa Fluor 647 NB110-13364AF647
Alexa Fluor 700 NB110-13364AF700
Alexa Fluor 750 NB110-13364AF750
Biotin NB110-13364B
DyLight 350 NB110-13364UV
DyLight 405 NB110-13364V
DyLight 488 NB110-13364G
DyLight 550 NB110-13364R
DyLight 594 NB110-13364DL594
DyLight 650 NB110-13364C
DyLight 680 NB110-13364FR
DyLight 755 NB110-13364IR
HRP NB110-13364H
Janelia Fluor 549 NB110-13364JF549
Janelia Fluor 646 NB110-13364JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol RAD54L