Rad51C Antibody (3F3-5C6) - Azide and BSA Free

Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody (M01), clone 3F3-5C6.Lane 1: RAD51C transfected lysate(42.2 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of monoclonal antibody to RAD51C on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi ( Cat # H00005889-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody (M01), clone 3F3-5C6 (Cat # H00005889-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - RAD51C monoclonal antibody (M01), clone 3F3-5C6 Analysis of RAD51C expression in HeLa.

Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - RAD51C monoclonal antibody (M01), clone 3F3-5C6. Analysis of RAD51C expression in 293.

ELISA: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Detection limit for recombinant GST tagged RAD51C is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 3F3-5C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Rad51C Antibody (3F3-5C6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RAD51C (AAH00667.1, 1 a.a. ~ 134 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRGKTFRFEMQRDLVSFPLSPAVRVKLVSAGFQTAEELLEVKPSELSKEVGISKAEALETLQIIRRECLTNKPRYAGTSESHKKCTALELLEQEHTQGFIITFCSALDDILGGGVPLMKTTEICGAPGVGKTQL
• Localization: Nuclear
• Specificity: RAD51C - RAD51 homolog C (S. cerevisiae)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD51C
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rad51C Antibody (3F3-5C6) - Azide and BSA Free

• BROVCA3
• DNA repair protein RAD51 homolog 3
• FANCO
• MGC104277
• R51H3
• RAD51 homolog C (S. cerevisiae)
• RAD51 homolog C
• RAD51L2RAD51 (S. cerevisiae) homolog C
• RAD51-like protein 2
• yeast RAD51 homolog 3

Background

This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. This gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Rad51C Antibody (H00005889-M01). (Showing 1 - 1 of 1 FAQ).

1. I am interested in potentially purchasing your Rad51C antibody (3F3-5C6) to use for WB and ICC. Do you have application references for this antibody? I would like to see published images of its use for ICC before using it.
   • We do not have any publication citations for H00005889-M01 Rad51C (3F3-5C6) yet, however, one of our other best selling Rad51C antibody (2H11-6) (NB100-177) has been cited in at least 18 research publications that shows its usefulness in WB and ICC-IF applications.

Bioinformatics

• Gene Symbol: RAD51C
• Entrez:
  • 5889(Human)
• OMIM:
  • 602774(Human)