Rad51 Antibody (2E5-E5)

Western Blot: Rad51 Antibody (2E5-E5) [H00005888-M01] - Analysis of RAD51 expression in transfected 293T cell line by RAD51 monoclonal antibody (M01), clone 2E5-E5.Lane 1: RAD51 transfected lysate(37 KDa).Lane 2: Non-transfected lysate.

Western Blot: Rad51 Antibody (2E5-E5) [H00005888-M01] - RAD51 monoclonal antibody (M01), clone 2E5-E5 Analysis of RAD51 expression in HeLa.

ELISA: Rad51 Antibody (2E5-E5) [H00005888-M01] - Detection limit for recombinant GST tagged RAD51 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

Rad51 Antibody (2E5-E5) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: RAD51 (AAH01459, 1 a.a. ~ 242 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD
• Specificity: RAD51 - RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RAD51
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rad51 Antibody (2E5-E5)

• BRCC5
• HRAD51
• HsRad51
• HsT16930
• RAD51 (S. cerevisiae) homolog (E coli RecA homolog)
• RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
• RAD51 homolog A
• Rad51
• RAD51ABRCA1/BRCA2-containing complex, subunit 5
• RAD51L3
• RecA, E. coli, homolog of
• RECADNA repair protein RAD51 homolog 1
• RecA-like protein
• recombination protein A

Background

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAD51
• Entrez:
  • 5888(Human)
• OMIM:
  • 114480(Human)
• Uniprot:
  • AAH01459(Human)